表皮生长因子EGF与未成熟卵母细胞体外成熟的相关研究

发布时间：2018-05-13 03:34

本文选题：表皮生长因子 + 卵母细胞　；参考：《重庆医科大学》2005年硕士论文

【摘要】：目的:了解 EGF 对未成熟卵母细胞体外成熟、受精及胚胎发展的影响。方法:未成熟卵母细胞分别取自 hCG 刺激和未刺激的小鼠卵巢,将生殖泡(GV)期卵母细胞分别移入 EGF 浓度为 0(对照)、0.5、1、2.5、5 和 10ng/ml 的 Ham’s F10 培养液中进行体外培养,观察生殖泡破裂(GVBD)和第一极体(PB1)排出的情况。将达到 MⅡ期的卵母细胞进行体外受精,观察原核和胚胎发展情况。选择Ⅰ~Ⅱ级 4 细胞胚胎植入假孕第 1 天小鼠的输卵管,观察产仔情况。结果:hCG 刺激(G-PR)组和 hCG 未刺激(G-UPR)组的 GV 期卵丘-卵母细胞复合物(COC),在体外培养 12、16、20 和 24 小时 EGF浓度 1、2.5、5、10ng/ml 组 GVBD 发生率高于对照组(P0.05,P0.01);培养 20、24 和 36 小时 EGF 浓度 2.5、5 和 10ng/ml 组 PB1 排出率高于对照组(P0.05,P0.01),其中 EGF 浓度 5ng/ml 组的 GVBD、PB1 发生率最高(P0.01)。G-UPR 组 GVBD 和 PB1 发生率的峰时间分别在20~24 小时和 24~36 小时;G-PR 组则分别发生在 12~16 小时和 20~24小时。两组 COC 在 1、2.5、5、10ng/ml EGF 浓度培养下的退化率分别低于对照组(P0.05,P0.01)。G-UPR 组和 G-PR 组卵母细胞的体外受精率、2 细胞和 4 细胞胚胎的发生率在 EGF 浓度为 5ng/ml 时明显高于对照组,差异有显著性意义(P0.01)。两组胚胎植入母体均有外观正常的活仔产出。结论:本研究显示 EGF 不仅可以促进 GV 期卵母细胞的成熟,对其受精能力和胚胎发展也有促进作用,而且可以抑制卵母细胞成熟过程中的凋亡、促进卵冠-丘颗粒细胞的扩展;EGF 的促进作用与剂量有关;使用促性腺激素加快了卵母细胞的体外成熟过程,同时也增加了卵母细胞的体外受精率。
[Abstract]:Objective : To investigate the effects of EGF on the in vitro maturation , fertilization and embryo development of immature oocytes .

Methods : The oocytes from immature oocytes were taken from hCG - stimulated and unstimulated mouse ovaries , and the oocytes were cultured in vitro to observe the growth of reproductive cells ( GVBD ) and the first polar body ( PB1 ) .

Results : The incidence of GVBD and PB1 in G - UPR group was higher than that in control group ( P0.05 , P0.01 ) . The rates of GVBD and PB1 in group G - UPR group were higher than those in control group ( P0.05 , P0.01 ) . In vitro fertilization rate , 2 - cell and 4 - cell embryos in G - UPR group and G - PR group were significantly higher when EGF concentration was 5 ng / ml ( P0.01 ) .

Conclusion : EGF can not only promote the maturation of GV oocytes , but also to the development of oocytes .

The fertilization ability and the embryo development also promote the development of the embryo , and can inhibit the apoptosis in the maturation of the oocyte and promote the extension of the egg - crown - Chuqiu granular cell ; the promotion effect of EGF is related to the dose ; the in vitro maturation process of the oocyte is accelerated by using the gonadotropins , and the in vitro fertilization rate of the oocyte is also increased .

【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R321

【参考文献】