肺炎克雷伯杆菌粘附作用与细菌毒力相关性的初步探讨

发布时间：2018-05-13 03:42

本文选题：呼吸道上皮细胞 + 肺炎克雷伯杆菌　；参考：《四川大学》2006年硕士论文

【摘要】：肺炎克雷伯杆菌是常见的医院内感染机会性致病菌，，它可导致尿路感染，也可造成严重的细菌性肺炎。肺炎克雷伯杆菌所致的细菌性肺炎进展快，临床表现复杂，抗生素治疗往往不能及时产生疗效，有较高的致死率。而肺炎克雷伯杆菌对多种抗生素耐药的现象也成为治疗的难点。本研究旨在探讨两个问题：1．肺炎克雷伯杆菌对呼吸道上皮细胞粘附能力与肺炎克雷伯杆菌对小鼠的毒力之间的相互关系。2．人中性粒细胞α-防御素对肺炎克雷伯杆菌肺部感染模型动物的保护作用及可能的机制。首先肺炎克雷伯杆菌临床分离株K4、K5、K7、K10分别与人肺Ⅱ型上皮细胞株A549(使细菌与细胞比例为100：1)共孵育2小时。PBS清洗未粘附的细菌，TritonX-100碎裂上皮细胞，松解释放粘附的肺炎克雷伯杆菌。收集裂解混合液，适当稀释后LB培养板涂布，培养过夜，计数并分析比较肺炎克雷伯杆菌各菌株粘附上皮细胞的差异。雄性昆明小鼠随机分成4组，每组10只。1.5％戊巴比妥钠腹腔注射麻醉准备。对数生长期肺炎克雷伯杆菌K4、K5、
[Abstract]:Klebsiella pneumoniae is a common opportunistic pathogen in nosocomial infection , which can lead to urinary tract infection , and can cause severe bacterial pneumonia . Bacterial pneumonia caused by Klebsiella pneumoniae is rapid , clinical manifestation is complicated , and antibiotic therapy often fails to produce the therapeutic effect in time . The relationship between the adhesion ability of Klebsiella pneumoniae to respiratory epithelial cells and the virulence of Klebsiella pneumoniae to mice . Protective effect and possible mechanism of human neutrophil alpha - defensins against Klebsiella pneumoniae lung infection model animals .

First , the clinical isolates of Klebsiella pneumoniae were incubated for 2 hours with human lung II epithelial cell line A549 ( the ratio of bacteria to cells was 100 : 1 ) for 2 hours .

【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R378

【共引文献】