缺陷LB膜诱导草酸钙晶体生长

发布时间：2018-05-14 02:02

本文选题：缺陷LB膜 + 草酸钙　；参考：《暨南大学》2006年硕士论文

【摘要】：本论文首次采用草酸钾损伤形成的缺陷LB膜作为受损伤肾上皮细胞膜的模型，采用扫描电子显微镜(SEM)、原子力显微镜(AFM)、X射线衍射(XRD)等表征方法，从分子和超分子的水平上研究LB膜缺陷的微结构及其对CaOxa晶体成核、生长和沉积图形的影响。 DPPC LB膜经草酸钾处理后形成的缺陷LB膜能诱导生成大量圈状—水草酸钙(COM)晶体图形，相比之下，没有经草酸钾处理的LB膜只诱导零散的六边形COM晶体。构成圈状图形的草酸钙晶体主要以针状COM的为主，其原因是由LB膜中的圈状缺陷诱导所致。缺陷LB膜在0.3mmol／L的草酸钙亚稳溶液中生长20分钟就可观测到圈状晶体图形，而常规LB膜在1小时后才有晶体，普通水溶液要6小时，促进COM晶体的异相成核的强度依次为：缺陷LB膜＞常规LB膜＞无LB膜水溶液。生长时间影响圈状图形的形貌和数量。随着生长时间增加，空心的圈状COM晶体图形转变为实心的圆形晶体图形，不同尺寸的实心圈状晶体图形的比例增加，圈内单个COM晶体的尺寸明显增大。同时小尺寸(5-20μm)的圈状晶体图形比例增加，而大尺寸(＞50μm)的圈状图形比例减少，中等尺寸的(20-50μm)的没有明显的变化。损伤LB膜的草酸钾浓度对圈状晶体图形有明显影响。随着草酸盐浓度的增加，，圈状晶体图形里面的COM变得越来越稀少。图形由0.3mmol／L时的实心圆形逐渐变成5.0mmol／L的环状晶体图形。圈状图形里面的COM数量为0.3＞0.6＞2.5＞5.0。同时圈状晶体图形的数量增多，其规律为：5.0＞2.5＞0.6＞0.3。增加LB膜在干燥器中放置时间，缺陷LB膜能诱导生成带孔的圈状草酸钙晶体图形。归因于LB膜中的DPPC分子重排，造成堆积结构发生变化。这些结果有助于在分子和超分子的水平上阐明肾上皮细胞膜损伤后诱导肾结石形成的的分子机制。
[Abstract]:In this paper, the defective LB film formed by potassium oxalate injury was used as the model of the injured renal epithelial cell membrane for the first time, and characterized by scanning electron microscopy (SEM) and atomic force microscope (AFM) X-ray diffraction (XRD). The microstructures of LB film defects and their effects on the nucleation growth and deposition patterns of CaOxa crystals were studied at molecular and supramolecular levels. The defective LB films formed by the treatment of DPPC LB films with potassium oxalate can induce a large number of circular calcium oxalate crystal patterns, whereas the LB films without potassium oxalate treatment can only induce scattered hexagonal COM crystals. The crystal of calcium oxalate is mainly composed of needle-like COM, which is caused by the ring defect in LB film. The defect LB film can be observed in the metastable solution of calcium oxalate of 0.3mmol/L for 20 minutes, whereas the conventional LB film has a crystal only after 1 hour, and the ordinary aqueous solution takes 6 hours. The intensity of heterogeneous nucleation of COM crystals is in the order of defect LB film > conventional LB film > aqueous solution without LB film. Growth time affects the shape and number of ring shapes. With the increase of growth time, the shape of hollow annular COM crystal is transformed into a solid circular crystal pattern, and the proportion of solid ring crystal pattern of different sizes increases, and the size of a single COM crystal increases obviously. At the same time, the proportion of ring shape of small size (5-20 渭 m) increased, while that of large size (> 50 渭 m) decreased, while that of medium size (20-50 渭 m) did not change obviously. The concentration of potassium oxalate damaged LB film has a significant effect on the shape of ring crystal. With the increase of oxalate concentration, the COM in the ring crystal pattern becomes more and more rare. The figure changes from the solid circle of 0.3mmol/L to the ring crystal pattern of 5.0mmol/L. The number of COM in the circle figure is 0. 3 > 0. 6 > 2. 5 > 5. 0. At the same time, the number of ring crystal figures increased, and the rule was: 1: 5.0 > 2.5 > 0.6 > 0.3. When the LB film is placed in the drier, the defective LB film can induce the formation of a circular crystal pattern of calcium oxalate with holes. Due to the rearrangement of DPPC molecules in LB films, the stacking structure changes. These results are helpful to elucidate the molecular mechanism of renal stone formation induced by membrane damage of renal epithelium at molecular and supramolecular levels.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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