CpG寡核苷酸增强重组HBsAg疫苗免疫应答的实验研究

发布时间：2018-05-17 12:47

本文选题：CpG + ODN　；参考：《大连医科大学》2006年硕士论文

【摘要】： 目的通过一些体内和体外试验证实,来自细菌或人工合成的非甲基化的CpG寡核苷酸(ODN)能诱导免疫淋巴细胞增殖及免疫球蛋白、单核细胞因子、INF-γ等的分泌,并能激活自然杀伤细胞的溶细胞效应。CpG ODN的这些功能意味它可能在增强机体对疫苗的免疫应答方面具有一定的前景。现有的HBsAg重组蛋白疫苗虽说在一定程度上能起到较好的作用,但一些调查统计表明,在经过此种疫苗接种的人群中,约有10%~15%的免疫人群并未产生较理想的免疫效果。正因为如此,许多研究人员正试图从新型免疫佐剂的开发这一方向寻找克服这个缺点的新型疫苗。本实验就是围绕这一主题试图通过CpG寡核苷酸(CpG ODN)与乙型肝炎病毒重组蛋白疫苗联合免疫C57BL/6小鼠,观察它对小鼠免疫状况的影响。进而探讨CpG寡核苷酸(CpG ODN)能否作为一种佐剂与HBsAg疫苗一起,更好地刺激机体免疫系统产生有效的应答。方法将小鼠分为三组,第一组为生理盐水对照,第二组为乙型肝炎病毒重组蛋白疫苗接种,第三组为CpG ODN与重组HBsAg疫苗联合接种,按照第1天、第15天、第22天的程序进行免疫接种,于免疫后第29天分别采血1.0ml,并处死小鼠迅速制备脾细胞悬液。用MTT法分别进行HBsAg蛋白特异性刺激的淋巴细胞转化试验、HBsAg蛋白特异性刺激的细胞毒性T细胞杀伤试验(HBsAg specific
[Abstract]:Objective to study in vitro and in vivo studies that CpG oligodeoxynucleotides (ODNs) from bacteria or synthetic unmethylated CpG can induce the proliferation of immune lymphocytes and the secretion of immunoglobulin, monocyte cytokines, such as INF- 纬, etc. These functions of CpG ODN, which can activate the cytotoxic effect of natural killer cells, may have a certain prospect in enhancing the immune response to the vaccine. Although the existing HBsAg recombinant protein vaccine can play a better role to some extent, some surveys and statistics show that about 10% or 15% of the population vaccinated with this vaccine did not have an ideal immune effect. Because of this, many researchers are trying to find new vaccines to overcome this shortcoming in the direction of developing new immune adjuvants. The purpose of this study was to immunize C57BL/6 mice with recombinant protein vaccine of hepatitis B virus by CpG oligodeoxynucleotide (CpG ODN) and to observe its effect on the immune status of mice. To explore whether CpG oligonucleotide can be used as an adjuvant with HBsAg vaccine to stimulate the immune system to produce an effective response. Methods mice were divided into three groups, the first group was saline control group, the second group was hepatitis B virus recombinant protein vaccine, the third group was CpG ODN combined with recombinant HBsAg vaccine, according to the 1st day, 15th day. On the 29th day after immunization, 1.0 ml blood was collected, and mice were killed to prepare spleen cell suspension. Lymphocyte transformation test stimulated by HBsAg protein by MTT method and cytotoxic T cell killing test stimulated by HBsAg protein
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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