NGF，TrkA，P75NTR在人胚胎结肠神经系统中的表达与分布

发布时间：2018-05-18 03:38

本文选题：肠神经系统 + 神经生长因子　；参考：《安徽医科大学》2006年硕士论文

【摘要】：目的研究人胚胎结肠神经系统的发育过程中神经生长因子(Nerve Growth Factor，NGF)与其两个受体TrkA(tyrosine kinase A)、P75NTR(Nerve Growth Factor low affinity receptor P75)在结肠神经系统中的分布及与结肠神经系统发育的关系。方法采用一抗为PGP9.5(protein gene product 9.5)、NGF、TrkA、P75NTR的免疫组化技术，显示结肠神经系统不同发育阶段肠壁中的神经元。结果人胚胎发育有明显的阶段性，在不同的阶段神经生长因子(NGF)、高亲和力受体TrkA、低亲和力受体P75NTR在神经细胞中的表达水平不同。在胚胎发育早期(胎龄4个月以前)，肠壁发育差，以后出现菲薄的平滑肌层和低平的肠粘膜，偶见NGF免疫反应性神经胶质细胞；至发育中期(胎龄4-5个月)，肠壁分化出4层结构，出现相当发达绒毛，NGF、TrkA、P75NTR、PGP9.5免疫反应性细胞明显增多，弥散分布于整个肌层间并逐渐迁移到粘膜下层和粘膜层；至发育晚期(6-9个月)，肠壁各层均增厚，，肌间神经丛成簇分布，神经纤维构成的网络出现更为细小的3级突起，粘膜下神经丛分化形成浅丛和深从；NGF在胞浆、突起，TrkA在胞浆、胞膜、胞核上，PGP9.5在胞浆上着色进一步加深，免疫反应进一步增强；P75NTR免疫反应阳性细胞数增多但着色深浅并无明显变化，染色强度变化不大。结论结肠神经系统的发育有明显的阶段性。在发育早期未见NGF、TrkA、P75NTR、PGP9.5免疫反应性神经细胞；在发育中期NGF、TrkA、P75NTR、PGP9.5神经成分在肠壁各层中出现并发育增生；晚期肠壁各层神经成分分化成熟，NGF、TrkA、PGP9.5染色强度进一步增强，但P75NTR染色强度变化不大；在神经系统发育不同阶段，NGF、TrkA与P75NTR比例在发生变化，在不同的发育阶段P75NTR和NGF、TrkA相互作用发挥不同的生理学效应，而在不同的发育阶段，原始病因引起NGF、TrkA、P75NTR之间比例异常，将导致肠神经发育异常。
[Abstract]:Objective to study the distribution of nerve growth factor (NGF) and its two receptors, TrkA(tyrosine kinase Agna P75NTRNerve Growth Factor low affinity receptor P75, in the colonic nervous system during the development of the colonic nervous system of human embryo and its relationship with the development of the colonic nervous system. Methods Immunohistochemical technique was used to display neurons in the intestinal wall of the colon nervous system at different stages of development. Results there were obvious stages in the development of human embryos. The expression levels of NGF, TrkA and P75NTR in nerve cells were different at different stages. NGF immunoreactive glial cells were occasionally found in the early stage of embryonic development (4 months before gestational age, with poor development of intestinal wall, thin smooth muscle layer and low level intestinal mucosa). At the middle stage of development (4-5 months of gestational age), the intestinal wall differentiated into four layers, and the immunoreactive cells of NGF TrkAn P75NTRN PGP9.5 increased obviously, distributed throughout the myometrium and gradually migrated to the submucous and mucous layers. In the late stage of development, every layer of the intestinal wall was thickened, the intermuscular nerve plexus was clustered, the network of nerve fibers formed a smaller three-grade process, and the submucosal plexus differentiated into superficial plexus in the cytoplasm, and the neurite TrkA in the cytoplasm, and the submucosal plexus differentiated into a superficial plexus in the cytoplasm and TrkA in the cytoplasm. The staining of PGP 9.5 on the membrane and nucleus was further deepened, and the immunoreaction was further enhanced. The number of P75NTR immunoreactive positive cells increased, but the staining depth did not change obviously, and the staining intensity did not change much. Conclusion the development of the colon nervous system has obvious stages. In the early stage of development, there were no immunoreactive nerve cells of NGF TrkAn P75NTRN PGP9.5; in the middle stage of development, the neuronal components of NGF TrkAgna P75NTRN PGP9.5 appeared and proliferated in all layers of the intestinal wall, and the staining intensity of NGFTK TrkAg PGP9.5 was further enhanced in the late stage of the differentiation and maturation of the nerve components of the various layers of the intestinal wall. However, the intensity of P75NTR staining did not change significantly, and the ratio of P75NTR TrkA to P75NTR changed in different stages of nervous system development. The interaction between P75NTR and NGF TrkA played different physiological effects in different developmental stages, but in different developmental stages. The original etiology causes abnormal ratio between NGFV TrkAgna P75 NTR and leads to abnormal development of intestinal nerve.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R321

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