沈阳KM小鼠源和长春牛源隐孢子虫卵囊形态的数字图像分析

发布时间：2018-05-18 18:31

本文选题：隐孢子虫 + 卵囊　；参考：《中国医科大学》2006年硕士论文

【摘要】：目的隐孢子虫(Cryptosporidium)是一种呈世界性分布的机会性致病原虫,已被列为导致人体腹泻的6种病原体之一。因其引起的水源性及食源性腹泻的爆发性流行及可成为AIDS患者、免疫力低下儿童的致死性病因而倍受重视。国外已将隐孢子虫作为AIDS患者必检的项目之一。卵囊是隐孢子虫主要的病原学诊断依据。不同动物源的隐孢子虫卵囊在形态大小及致病性上有一定差异,因此隐孢子虫卵囊的形态学研究对分类、致病性及流行病学研究具有一定的意义。有关隐孢子虫卵囊的形态及大小已有一些描述,但至今尚未见采用数字图像处理技术分析隐孢子虫卵囊形态学参数的报道,也没有见到用该技术对不同动物源隐孢子虫卵囊进行对比分析的资料。与传统的形态学研究方法相比,数字图像处理技术可以更客观、精确、灵活、大通量地获取信息,且因具有快速、简捷、易于操作等优点而倍受青睐。近年来,该技术已被用于一些寄生虫的形态学研究。本研究采用数字图像分析技术建立并分析隐孢子虫卵囊的形态学参数,对比分析了沈阳地区小鼠源和长春地区牛源隐孢子虫卵囊的形态大小,从数理分析角度确定了隐孢子虫卵囊的形态学差异。本研究结果可为隐孢子虫的病原学及流行病学研究提供更可靠的形态学资料。材料与方法 1.隐孢子虫卵囊的来源 1.1 沈阳地区KM小鼠源隐孢子虫卵囊:中国医科大学实验动物部培育的昆明株(KM)小鼠,经地塞米松免疫抑制处理,诱导小鼠隐孢子虫感染,建立小鼠隐孢子虫感染模型。取小鼠粪便经涂片抗酸染色检获到隐孢
[Abstract]:Purpose Cryptosporidium (Cryptosporidium), a worldwide opportunistic protozoa, has been listed as one of the six pathogens leading to human diarrhea. Due to the outbreak of waterborne diarrhea and food-borne diarrhea, which can become the fatal cause of AIDS patients and children with low immunity, much attention has been paid to it. Cryptosporidium has been regarded as one of the necessary screening items for AIDS patients abroad. Oocysts are the main basis for etiological diagnosis of Cryptosporidium. The morphology and pathogenicity of Cryptosporidium oocysts from different animal sources are different, so the morphological study of Cryptosporidium oocysts has some significance for classification, pathogenicity and epidemiology. The morphology and size of Cryptosporidium oocysts have been described, but no digital image processing technique has been reported to analyze the morphological parameters of Cryptosporidium oocysts. There was no comparative analysis of Cryptosporidium oocysts from different animal sources by using this technique. Compared with the traditional morphological research methods, digital image processing technology can be more objective, accurate, flexible, large throughput of information acquisition, and has the advantages of fast, simple, easy to operate and so on. In recent years, this technique has been used to study the morphology of some parasites. In this study, the morphological parameters of Cryptosporidium oocysts were established and analyzed by digital image analysis, and the morphological size of Cryptosporidium oocysts in Shenyang and Changchun were compared. The morphological differences of Cryptosporidium oocysts were determined from the point of view of mathematical analysis. The results can provide more reliable morphological data for etiology and epidemiology of Cryptosporidium. Materials and methods 1. Source of Cryptosporidium oocysts 1.1 Cryptosporidium oocysts of km mice in Shenyang area: Kunming strain KM (Kunming strain KM) cultured in laboratory animal department of China Medical University was treated with dexamethasone immunosuppressive treatment to induce Cryptosporidium infection in mice and to establish the model of Cryptosporidium infection in mice. Seizure of Cryptosporium from mouse feces by acid fast staining
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R382.9

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