MAPK和ROS在慢性饮酒大鼠胃黏膜细胞增殖中作用的研究

发布时间：2018-05-19 04:24

本文选题：酒精 + 胃黏膜　；参考：《南京医科大学》2006年硕士论文

【摘要】：以往的研究证实，慢性饮酒可使胃黏膜细胞更新加快，引起胃黏膜适应性细胞保护作用，但其机制尚不清楚。本研究在以往建立的慢性饮酒动物模型的基础上，，探讨长期摄入低浓度酒精和胃黏膜细胞增殖之间的相关性以及与此相关的分子事件，尤其关注活性氧(reactive oxygen species，ROS)及细胞外信号调节激酶(extracellular signal-regulated kinases，ERKs)、大分子MAPK(big mitogen-activated protein kinases-1，BMK1)和c-Jun氨基末端激酶(c-Jun N-terminal kinase，JNK)信号通路是否参与了慢性饮酒引起的大鼠胃黏膜细胞增殖过程。将成年雄性SD大鼠随机分为四组，第一组(对照组)：饮用清水；第二组(酒精组)：饮用6％(v／v)酒精水溶液；第三组(槲皮素组)：饮用清水，槲皮素(100mg／kg)每12h灌胃一次；第四组(酒精+槲皮素组)：饮用6％酒精水溶液，同时每隔12h给予槲皮素(100mg／kg)灌胃一次，均处理7天。在处理7天末，取大鼠胃黏膜，用流式细胞术、增殖细胞核抗原(PCNA)的免疫组化染色以及PCNA和细胞周期蛋白D1(CyclinD1)的免疫印迹分析观察胃黏膜的细胞增殖情
[Abstract]:Previous studies have confirmed that chronic drinking can accelerate the regeneration of gastric mucosal cells and induce adaptive cell protection of gastric mucosa, but its mechanism is not clear. Based on the previous animal models of chronic drinking, the relationship between chronic alcohol intake and gastric mucosal cell proliferation and the related molecular events were investigated. In particular, Ros) and extracellular signal-regulated kineses (MAPK(big mitogen-activated protein kinases-1hBMK1) and c-Jun amino terminal kinase c-Jun N-terminal kinase (c-Jun) signaling pathway were involved in the proliferation of gastric mucosal cells induced by chronic drinking in rats. Adult male SD rats were randomly divided into four groups: the first group (control group: drinking clear water), the second group (alcohol group: drinking 6 v / v) alcohol aqueous solution, the third group (quercetin group: drinking water, quercetin 100 mg / kg) every 12 hours; The fourth group (alcohol quercetin group: drinking 6% alcohol aqueous solution, at the same time once every 12 hours, 100 mg / kg quercetin) was treated for 7 days. At the end of 7 days, gastric mucosa was taken from rats. The proliferation of gastric mucosa was observed by flow cytometry, immunohistochemical staining of proliferating cell nuclear antigen (PCNA) and immunoblotting analysis of PCNA and cyclin D1CyclinD1).
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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