念珠菌显色培养基的研制和临床评价

发布时间：2018-05-20 01:38

本文选题：念珠菌 + 念珠菌病　；参考：《郑州大学》2006年硕士论文

【摘要】： 研究背景及目的：念珠菌病是一类与滥用抗生素、机体免疫缺陷、妊娠、雌激素治疗、难治性糖尿病等因素密切相关的疾病。快速有效地检测和鉴别念珠菌对于念珠菌病的诊断和治疗具有重要意义，但目前临床上尚缺乏比较简单有效的方法，特别对于外阴阴道念珠菌病(vulvovaginal candidiasis，VVC)而言，由于使用的标本是阴道分泌物，其成分比较复杂，检测和鉴别念珠菌时较为困难。上世纪末国际上出现的念珠菌显色培养基一定程度上解决了这一难题，但国内在念珠菌显色培养基的研制方面还未见报道。进口受限、价格昂贵往往影响念珠菌显色培养基的实际使用，为了满足国内对念珠菌显色培养基的需求，，本课题利用酶显色底物研制念珠菌显色培养基并对其进行临床评价，以期获得具有较高敏感性、特异性、经济性的产品。方法：以沙保罗葡萄糖琼脂为基础，结合酶显色底物(H012、H026、H092)，通过一系列优化实验和两组正交实验，筛选出念珠菌显色培养基的最佳配方；再分别用166株临床分离的念珠菌菌株和212例临床VVC疑似患者标本，以CHROMagar念珠菌显色培养基作对照，评价该培养基的性能。结果：①研制的念珠菌显色培养基主要成分：日本琼脂、蛋白胨、麦芽浸膏、酵母浸膏、氯化血红素、葡萄糖、N-乙酰-D-氨基葡萄糖、H012、H026、H092、BB2、巯基乙醇和土霉素等。②用临床分离的念珠菌菌株评价，与CHROMagar念珠菌显色培养基没有显著性差异，P＞0.05。③用临床VVC疑似患者标本评价，与CHROMagar念珠菌显色培养基没有显著性差异，P＞0.05。结论：自行研制的念珠菌显色培养基在性能上与CHROMagar念珠菌显色培养基没有显著性差异，可用于临床检测。
[Abstract]:Background and objective: candidiasis is a class of diseases closely related to the abuse of antibiotics, immune deficiency, pregnancy, estrogen therapy, refractory diabetes and other factors. Rapid and effective detection and identification of candida is of great significance for the diagnosis and treatment of candidiasis, but there is a lack of simple and effective methods in clinical practice, especially for vulvovaginal candidiasis VVCC. Because the samples used are vaginal secretions, the composition of the complex, detection and identification of Candida is more difficult. At the end of last century, the Candida chromogenic medium appeared in the world has solved this problem to some extent, but the development of Candida chromogenic medium has not been reported in our country. In order to meet the demand of Candida color medium in our country, the enzyme substrate was used to develop the Candida chromogenic medium and evaluate it clinically, in order to satisfy the demand of Candida color medium, which is limited in import and expensive in price, in order to meet the domestic demand of Candida chromogenic medium, this paper developed the Candida color medium with enzyme substrate. In order to obtain products with high sensitivity, specificity and economy. Methods: on the basis of Sapor glucose Agar and combined with the enzyme substrate H012H026H092A, a series of optimized experiments and two groups of orthogonal experiments were carried out to select the optimum formula of the color medium for Candida. The performance of Candida albicans isolated from clinical samples and 212 suspected patients with clinical VVC was evaluated by using CHROMagar culture medium as control. Results the main components of Candida chromogenic medium: Japanese Agar, peptone, malt extract, yeast extract, hemin, hemin, Glucose N- acetyl D- glucosamine H012H026H092BB2, mercaptoethanol and oxytetracycline were evaluated by clinical isolates of Candida, and there was no significant difference with CHROMagar culture medium (P > 0. 05. 3). The samples of suspected patients with clinical VVC were used to evaluate. There was no significant difference between the culture medium of Candida CHROMagar and that of Candida CHROMagar (P > 0.05). Conclusion: there is no significant difference between the developed culture medium and the CHROMagar culture medium, which can be used for clinical detection.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R379

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