氟苯尼考残留对细菌耐药性的影响

发布时间：2018-05-20 20:09

本文选题：氟苯尼考 + 药物残留　；参考：《西南民族大学》2006年硕士论文

【摘要】： 为研究氟苯尼考残留对细菌耐药性的影响,本文采用含氟苯尼考的培养基模拟机体肠道内药物残留环境,进行了两部分试验: 试验一是在体外诱导三种人体肠道细菌——大肠杆菌、产气夹膜梭菌、粪肠球菌分别对氟苯尼考产生不同水平的耐菌性,然后采用二倍稀释法检测这些耐药菌株对氯霉素、头孢曲松、氨苄西林、四环素、土霉素、庆大霉素、阿米卡星、氧氟沙星、环丙沙星等常规药物敏感性变化。结果显示,对于不同的细菌,氟苯尼考与上述不同种药物存在不同程度的交叉耐药性。试验二是在体外诱导动物源性大肠杆菌O_(78)对氟苯尼考产生不同水平的耐药性,观察不同诱导时间和不同的药物浓度对耐药性性的影响。结果显示,对于同一诱导药物浓度,诱导时间越长,细菌耐药水平越高,但增长速度减慢,最后趋于一稳定水平。当诱导药物浓度为2μg/ml、64μg/ml、128μg/ml时,细菌耐药性增长速度慢,当诱导药物浓度为4μg/ml、8μg/ml、16μg/ml、32μg/ml时,细菌耐药性增长速度快。通过本试验结果,证实动物专用抗菌药物残留对人体存在一定影响,并影响人类临床用药。同时,还比较客观的观察了氟苯尼考耐药菌株的产生过程,比较了不同残留水平和残留时间对耐药性的产生的影响。这些结论,对于临床合理使用氟苯尼考,减少残留,延缓细菌耐药性增长速度,延长氟苯尼考临床使用寿命,具有一定参考价值。
[Abstract]:In order to study the effect of florfenicol residue on bacterial drug resistance, a two-part experiment was carried out in this paper, in which florfenicol containing florfenicol was used to simulate the environment of drug residues in intestinal tract. The first experiment was to induce in vitro three kinds of human intestinal bacteria, E. coli, Clostridium aerogenes and Enterococcus faecalis, to produce different levels of resistance to florfenicol, and then double dilution method was used to detect the resistance of these resistant strains to chloramphenicol. Changes in sensitivity of ceftriaxone, ampicillin, tetracycline, oxytetracycline, gentamicin, amikacin, ofloxacin and ciprofloxacin. The results showed that florfenicol had different cross-resistance to different bacteria. The second experiment was to induce different levels of drug resistance to florfenicol in vitro. The effects of different induction time and drug concentration on drug resistance were observed. The results showed that for the same drug concentration, the longer the induction time was, the higher the drug resistance level of bacteria was, but the growth rate slowed down and finally tended to a stable level. When the concentration of induced drug was 2 渭 g / ml, 64 渭 g / ml, 128 渭 g/ml, the drug resistance of bacteria increased slowly. When the concentration of induced drug was 4 渭 g / ml, 16 渭 g / ml or 32 渭 g/ml, the drug resistance of bacteria increased rapidly. The results show that animal-specific antimicrobial residues have a certain impact on human body and affect human clinical drug use. At the same time, the production process of florfenicol resistant strains was observed objectively, and the effects of different residue levels and residual time on drug resistance were compared. These conclusions have certain reference value for the rational use of florfenicol in clinic, reducing residues, delaying the growth rate of bacterial resistance and prolonging the clinical service life of florfenicol.
【学位授予单位】：西南民族大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R155.5;R378

【引证文献】