N-乙酰半胱氨酸减轻香烟烟雾提取物所致的A549细胞损伤

发布时间：2018-05-23 13:38

本文选题：IGFBP- + 香烟烟雾提取物　；参考：《基础医学与临床》2014年01期

【摘要】：目的研究N-乙酰半胱氨酸(NAC)对香烟烟雾提取物(CSE)暴露后的A549细胞的影响。方法 MTT法测定不同处理后A549细胞的存活率;用PI单染和AnnexinV-FITC/PI双染检测细胞的周期变化和凋亡变化;用荧光探针H2DCFDA检测细胞内活性氧簇(ROS)变化;用实时荧光定量PCR和Western blot技术检测胰岛素样生长因子结合蛋白-3(IGFBP-3)的表达变化。结果 CSE致A549细胞存活的A值为0.55±0.04,显著低于对照组的0.78±0.03(P0.05),NAC处理使A值显著上升至0.67±0.04(P0.05),并消除了G1期阻滞,降低细胞凋亡率等。IGFBP-3的表达在CSE刺激后,有所升高,在接受NAC保护后,其表达水平有所降低(P0.05)。结论 NAC可以抑制香烟烟雾提取物造成的A549细胞损伤,IGFBP-3可能在香烟烟雾提取导致细胞损伤的机制中发挥作用。
[Abstract]:Objective to study the effects of N-acetylcysteine (NACN) on A549 cells exposed to cigarette smoke extract (CSE). Methods MTT assay was used to detect the survival rate of A549 cells, Pi single staining and AnnexinV-FITC/PI double staining were used to detect the changes of cell cycle and apoptosis, and fluorescence probe H2DCFDA was used to detect the changes of reactive oxygen species (Ros) in A549 cells. The expression of IGFBP-3 in insulin-like growth factor binding protein (IGFBP-3) was detected by real-time quantitative PCR and Western blot. Results the A value of CSE induced A549 cell survival was 0.55 卤0.04, which was significantly lower than that of the control group (0.78 卤0.03P0.05). The A value of A549 cells was significantly increased to 0.67 卤0.04P0.05N, and the G1 phase arrest was eliminated, the apoptosis rate was decreased, and the expression of IGFBP-3 was increased after CSE stimulation, and the expression of IGFBP-3 was increased after NAC protection. The expression level of P0. 05 was decreased. Conclusion NAC can inhibit the injury of A549 cells induced by cigarette smoke extract. IGFBP-3 may play a role in the mechanism of cigarette smoke extraction.
【作者单位】：兰州大学基础医学院病理学研究所;
【基金】：兰州大学中央高校基本科研业务费专项资金(lzujbky-2009-92,lzujbky-2013-223)
【分类号】：R363

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