氯化镉对雄性大鼠颌下腺的影响及丙酸睾酮保护作用的研究

发布时间：2018-05-27 03:40

本文选题：颌下腺 + 镉　；参考：《重庆医科大学》2005年硕士论文

【摘要】：目的:探讨氯化镉对雄性大鼠颌下腺的影响及丙酸睾酮的保护作用。方法:雄性 Wistar 大鼠 35 只随机分为 3 组:正常对照组、单纯镉(Cd)组和镉加丙酸睾酮(Cd+T)组。Cd 组用 0.1% CdCl2(4mg/kg体重)腹腔内一次注射,Cd+T 组用 1%丙酸睾酮(5mg/kg x3)及 0.1%CdCl2(4mg/kg x1)腹腔内注射, 对照组给予等体积生理盐水。动物于处理后 24h、3、7、15、30d 取材并利用原子吸收火焰法、免疫组化 SABC法、透射电镜技术等作颌下腺组织镉含量测定,雄激素受体免疫组化半定量分析及颗粒曲管(GCT)细胞的光镜和超微结构观察。结果: (1)光镜下,镉注射后颌下腺形态无明显异常。电镜观察见镉注射后 24h,GCT 细胞内出现不同程度的线粒体肿胀和(或)空泡化,胞质和线粒体内可见髓样结构,GCT 周毛细血管内皮细胞线粒体肿胀。上述改变在镉注射后 7-15d 明显加重,并可见部分 GCT 细胞胞质广泛肿胀和轻度核溶解现象,30d 后 GCT 细胞仍未恢复正常形态。各时间点 Cd +T 组的超微结构损伤均较相应 Cd 组轻。(2)Cd 组和Cd+T 组的颌下腺组织镉含量在各时间点均明显高于对照组,并于 3-7d时达最高值,Cd+T 组的镉含量虽比相应时间点 Cd 组低,但两组比较差异无显著性。(3)AR 免疫反应产物分布在颌下腺腺泡、GCT、纹状管和排泄管细胞的胞浆和胞核,以胞浆为主。镉注射后 24h,AR 免疫反应产物减少,7d 时最低,至 30d 时仍低于对照组,Cd+T 组的 AR 免
[Abstract]:Objective: To investigate the effect of cadmium chloride on the submandibular gland of male rats and the protective effect of testosterone propionate.
Methods: 35 male Wistar rats were randomly divided into 3 groups: normal control group, simple cadmium (Cd) group and cadmium plus testosterone (Cd+T) group.Cd group with 0.1% CdCl2 (4mg/kg weight) intraperitoneal injection, Cd+T group using 1% testosterone propionate (5mg/kg x3) and 0.1%CdCl2 (4mg/kg x1) intraperitoneal injection, the control group was given equal volume of saline. Animals in the place. After 24h, 3,7,15,30d was obtained by atomic absorption flame method, immunohistochemistry SABC method and transmission electron microscope technique were used to determine the cadmium content in the submandibular gland tissue. The semi quantitative analysis of androgen receptor immunohistochemical staining and the light and ultrastructural observation of the granular convoluted tube (GCT) cells were observed.
Results: (1) under the light microscope, the morphology of the submandibular gland had no obvious abnormality after the injection of cadmium. It was observed in the electron microscope that the mitochondria swelling and (or) vacuolation were observed in 24h, GCT cells after cadmium injection, and the myeloid structure in the cytoplasm and mitochondria was visible, and the capillary endothelial cells of the capillary endothelial cells were swelling in the GCT weeks. The above changes were obviously aggravated after the injection of cadmium. There was extensive swelling and mild nucleation in the cytoplasm of some GCT cells, and the GCT cells did not return to normal form after 30d. The ultrastructural damage of Cd +T group at every time point was lighter than that of the corresponding Cd group. (2) the cadmium content in the submandibular gland of the Cd group and Cd+T group was significantly higher than that in the group at all time points, and reached the highest value at 3-7d, Cd+T. The cadmium content in the group was lower than that of the corresponding time point Cd group, but there was no significant difference in the two groups. (3) the AR immune reaction products were distributed in the submandibular gland acinus, GCT, the cytoplasm and nucleus of the striate and excretory cells, and the cytoplasm was the main cytoplasm. After the injection of 24h, the AR immunoreaction products decreased and the 7d was the lowest, and still lower than the control group and Cd+T group AR. Exempting
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R363

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