皮层星形胶质细胞Toll样受体4在炎症和β淀粉样蛋白形成中的作用（英文）

发布时间：2018-05-27 10:39

本文选题：阿尔茨海默病 + 星形胶质细胞　；参考：《生理学报》2014年06期

【摘要】：为了探讨星形胶质细胞在炎症和β淀粉样蛋白(amyloidβ-protein,Aβ)形成中的作用和可能的分子机制,本研究通过LPS刺激体外培养大鼠皮层星形胶质细胞,首先采用实时PCR和Western blot方法分别检测Toll样受体4(Toll-like receptor 4,TLR4)、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、白细胞介素-1β(interleukin 1β,IL-1β)、β淀粉样前体蛋白(β-amyloid precursor protein,β-APP)和β-位点APP剪切酶1(β-site APP clearing enzyme 1,BACE1)m RNA及TLR4、NF-κB/P65蛋白水平,而后用免疫荧光法进一步证明NF-κB/P65的核易位,ELISA法测定培养上清中TNF-α、IL-1β和Aβ含量。结果显示,这些指标在LPS刺激后均不同程度地上调。然而,如果预先用TLR4抗体处理,与仅用LPS刺激组相比,LPS对NF-κB/P65核易位及培养上清中TNF-α、IL-1β和Aβ含量的刺激作用显著减弱或消失。结果表明,星形胶质细胞TLR4可能通过TLR4/NF-κB信号通路在炎症和Aβ的形成中发挥重要的作用。
[Abstract]:In order to investigate the role of astrocytes in inflammation and the formation of amyloid 尾 -protein A 尾, and the possible molecular mechanism, the cultured rat cortical astrocytes were stimulated by LPS in vitro. At first, real-time PCR and Western blot were used to detect the protein levels of Toll receptor 4(Toll-like receptor 4, tumor necrosis factor 伪, interleukin-1 尾 -interleukin-1 尾, 尾 -amyloid precursor protein (尾 -APP), 尾 -site APP clearing enzyme 1BACE1mRNA and TLR4NF-魏 B/P65, respectively. Then immunofluorescence assay was used to further prove that the nuclear translocation Elisa method of NF- 魏 B/P65 was used to determine the contents of TNF- 伪 -nil IL-1 尾 and A 尾 in the culture supernatant. The results showed that these indexes were up-regulated in varying degrees after LPS stimulation. However, if TLR4 antibody was pretreated, the stimulation effect of TLR4 on the nuclear translocation of NF- 魏 B/P65 and the contents of TNF- 伪 -IL-1 尾 and A 尾 in the culture supernatant was significantly decreased or disappeared compared with the group stimulated by LPS alone. The results suggest that TLR4 may play an important role in inflammation and A 尾 formation through TLR 4 / NF- 魏 B signaling pathway.
【作者单位】：南通大学医学院病理生理学系;南京医科大学附属常州市第二人民医院病理科;南通大学医学院循证医学中心;
【基金】：supported by the Applied Research and Technology Program of Nantong Municipality,Jiangsu Province,China(No.BK2013007) the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)(2011),Jiangsu Province,China
【分类号】：R363

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