乙肝病毒X蛋白对肝细胞β-catenin的影响及其作用机制的研究

发布时间：2018-05-28 17:51

本文选题：乙肝病毒X蛋白 + β-连环蛋白　；参考：《重庆医科大学》2006年硕士论文

【摘要】： 乙型肝炎病毒(hepatitis B virus,HBV)编码的X蛋白在HBV致癌过程中发挥着关键性的作用。HBX作为反式作用因子,不能直接和双链DNA结合,而是通过蛋白与蛋白间的相互作用行使功能,可以调节转录、引起DAN修复蛋白质失活以及激活信号转导通路。Wnt信号传导途径在胚胎发育和肿瘤发生过程中起着十分重要的作用,而β-连环蛋白(β-catenin)是这个信号传导途径中的一个重要成员。HBX和β-catenin关系的研究对HBV导致肝癌的发生机制、肝癌的预防和治疗都有重要意义,为阐明Wnt信号转导途径中β-catenin在肝癌发生和发展中的作用提供理论依据。本课题采用带有GFP的腺病毒高表达载体系统有效转染人正常肝细胞系L02,并用Western blot证实HBX是否能有效在L02细胞中表达。用RT-PCR、免疫细胞化学方法分析HBx对正常细胞中β-catenin定位及基因表达的变化,并通过免疫共沉淀的方法来分析HBX与β-catenin、HBX与APC蛋白之间是否存在直接相互作用,了解HBX对正常肝细胞中β-catenin的影响及作用机制,为进一步研究HBX导致肝癌的机制及其预防治疗奠定基础。
[Abstract]:The X protein encoded by hepatitis B virus (HBV) plays a key role in the carcinogenesis of HBV. As a trans-acting factor, HBX does not bind directly to double-stranded DNA, but functions through the interaction between protein and protein. It can regulate transcription, cause DAN repair protein inactivation and activate signal transduction pathway. Wnt signal transduction pathway plays an important role in embryonic development and tumorigenesis. 尾 -catenin is an important member of this signaling pathway. The study of the relationship between HBX and 尾 -catenin plays an important role in the pathogenesis, prevention and treatment of hepatocellular carcinoma induced by HBV. In order to elucidate the role of 尾 -catenin in the pathogenesis and development of hepatocellular carcinoma in Wnt signal transduction pathway. In this study, adenovirus high expression vector system with GFP was used to transfect human normal liver cell line L02, and Western blot was used to confirm whether HBX could be expressed effectively in L02 cells. The changes of 尾 -catenin localization and gene expression in normal cells were analyzed by RT-PCR and immunocytochemistry, and the direct interaction between HBX and 尾 -catenin HBX and APC protein was analyzed by immunoprecipitation. To understand the effect and mechanism of HBX on 尾 -catenin in normal hepatocytes, and to lay a foundation for further study on the mechanism of HBX induced liver cancer and its prevention and treatment.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R373

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