P2受体对大鼠胃肠道平滑肌运动的调节作用

发布时间：2018-05-29 20:10

本文选题：ATP + 腺苷　；参考：《河北医科大学》2005年硕士论文

【摘要】：目的:观察腺苷三磷酸(ATP)对大鼠离体胃平滑肌运动的调节作用;观察ATP对大鼠离体近端结肠纵行肌运动的影响并探讨其受体机制;分析ATP介导大鼠胃体环行肌收缩反应的受体机制。方法:制备大鼠离体胃体纵行肌、胃体环行肌、胃窦纵行肌、胃窦环行肌和近端结肠纵行肌标本,观察ATP的作用;观察观察ATP、α,β-MeATP和腺苷对大鼠胃体环行肌的作用并分析相关的受体。结果:在胃体纵行肌标本,ATP(0.1 μmol·L~(-1) -1mmol·L~(-1))诱发微弱的舒张反应,继而出现收缩反应;在高钾预收缩条件下,ATP则引起较大舒张反应后续较小的收缩反应。ATP(0.1 μmol·L~(-1)-1 mmol·L~(-1))对胃体环行肌诱发单相收缩反应。低浓度ATP(1 μmol·L~(-1) -100 μmol·L~(-1))抑制胃窦纵行肌的自发性收缩幅度,同时加快收缩频率;高浓度ATP(1 mmol·L~(-1))则完全抑制自发性收缩反应;在高钾预收缩条件下,ATP产生浓度依赖性舒张反应。低浓度ATP(1 μmol·L~(-1)-10 μmol·L~(-1))先增大后减低胃窦环行肌的自发性收缩幅度,同时加快收缩频率;高浓度ATP(100 μmol·L~(-1)-1 mmol·L~(-1))则抑制自发性收缩反应。在静息张力时,ATP(0.1 μmol·L~(-1)-1 mmol·L~(-1))诱
[Abstract]:Aim: to observe the effects of adenosine triphosphate (ATP) on the motility of isolated rat gastric smooth muscle, to observe the effect of ATP on the motility of the longitudinal muscle of rat proximal colon and to explore the mechanism of its receptor. Objective: to investigate the receptor mechanism of ATP mediated gastric circular muscle contraction in rats. Methods: the specimens of isolated longitudinal muscle of gastric body, circular muscle of gastric body, longitudinal muscle of gastric antrum, circular muscle of gastric antrum and longitudinal muscle of proximal colon were prepared and the effect of ATP was observed. The effects of ATP, 伪, 尾 -MeATP and adenosine on the circular muscle of gastric body in rats were observed and the related receptors were analyzed. Results: in the longitudinal muscle of gastric body, ATP 0.1 渭 mol / L ~ (-1) -1mmol / L ~ (-1) induced a weak diastolic response and then a contractile response. Under the condition of high potassium precontraction, ATP induced a larger diastolic response, followed by a smaller contractile response. ATP-0.1 渭 mol / L ~ (-1) / L ~ (-1) mmol / L ~ (-1) induced monophasic contraction of the circular muscle of the gastric body. Low concentration of ATP(1 渭 mol L ~ (1) -100 渭 mol / L ~ (-1) inhibited the spontaneous contraction of the longitudinal muscle of gastric antrum and accelerated the contractile frequency, while the high concentration of ATP(1 mmol was completely inhibited the spontaneous contraction, and the concentration of ATP produced a concentration-dependent relaxation under the condition of high potassium precontraction. Low concentration of ATP(1 渭 mol L ~ (1) -10 渭 mol 路L ~ (-1) increased at first, then decreased the amplitude of spontaneous contraction of the antral circular muscle and increased the contractile frequency at the same time, while the high concentration of ATP(100 渭 mol L ~ (1) ~ (-1) mmol L _ (1) inhibited the spontaneous contraction. Inducement of ATP 0.1 渭 mol / L ~ (1) ~ (-1) mmol / L ~ (1) ~ (1) during resting tension
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R33

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