犬双肺序贯性移植模型的研究

发布时间：2018-05-31 07:20

本文选题：肺移植 + 犬　；参考：《中南大学》2007年硕士论文

【摘要】： 【目的】探讨犬双肺序贯性移植时，供肺的采取，保护及受体移植手术技巧及模型建立。【方法】首先建立犬双肺序贯性移植的动物模型：犬16条，，体重差别＜5％的随机配对，分供体及受体组。进行双肺序贯移植实验共8次，移植右肺钳夹左房时采用了‘v’钳夹技术，而后通过对左肺及右肺术后0.5h、1h、2h各进行PaO2、mPAP、HR、肺水含量的检测及电镜观察肺组织超微结构，了解术后肺功能情况。【结果】因心功能衰竭死亡两例，而后采取新的移植技术后良好的解决了在钳夹左房时因回心血量受阻而引起的心功能不全。mPAP术前术后有显著差异(P＜0.05)，而PaO2、肺水含量术前术后并无明显差异(P＞0.05)，表明移植后肺功能良好。【结论】掌握了犬双肺序贯性移植的全过程，“V”形双阻断钳夹左心房技术提高了犬肺移植的成功率。
[Abstract]:Objective: to investigate the surgical techniques and models of donor lung transplantation in dogs. [methods] Sixteen dogs were randomly matched and divided into donor group and recipient group. The bilateral lung sequential transplantation was carried out for 8 times. The right lung clamp technique was used when the right lung was grafted into the left atrium. Then, the left lung and the right lung were treated with PaO2 mPAPP HRA at 0.5 h and 2 h after operation respectively. The lung water content was measured and the ultrastructure of lung tissue was observed by electron microscope. To understand the pulmonary function after operation. [results] two patients died of heart failure, After adopting the new transplantation technique, the cardiac insufficiency caused by the obstruction of left atrial volume in the left atrium was well solved before and after operation (P < 0.05), but there was no significant difference in Pao _ 2 and lung water content before and after operation (P > 0.05), which indicated that the cardiac insufficiency caused by the obstruction of left atrium volume was not significantly different after operation (P > 0.05), which indicated that there was no significant difference in Pao _ 2 and lung water content after operation (P > 0.05). After transplantation, the lung function is good. [conclusion] the procedure of sequential bilateral lung transplantation was mastered, and the success rate of canine lung transplantation was improved by "V" double clamping of left atrium.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332;R655.3

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