S-亚硝基-N-乙酰半胱氨酸对绿脓菌素感染鼠体内的病理形态及氧化作用的影响

发布时间：2018-06-01 16:35

本文选题：绿脓菌素 + 乙酰半胱氨酸　；参考：《中国全科医学》2014年09期

【摘要】：目的探讨S-亚硝基-N-乙酰半胱氨酸(SNAC)对绿脓菌素(PCN)感染鼠体内的病理形态及氧化作用的影响。方法 90只健康雄性SD大鼠采用随机数字表法分为3组,每组30只。对照组和PCN组大鼠分别腹腔注射1 ml/100 g 0.9%氯化钠溶液,SNAC组大鼠腹腔注射SNAC(1 ml/100 g)。用1%戊巴比妥钠溶液(40 mg/kg)行大鼠腹腔注射麻醉,对照组气管内接种0.9%氯化钠溶液0.2 ml,PCN组和SNAC组分别气管内接种PCN 0.2 ml(6×108CFU/ml)。分别于6、18、30、48、72 h观察大鼠一般状态,处死大鼠,取支气管肺泡灌洗液(BALF)和肺组织,观察肺组织形态学改变,计算BALF细胞总数和中性粒细胞计数,检测活性氧(ROS)含量、超氧化物歧化酶(SOD)活性、白介素8(IL-8)含量。结果 PCN组鼠感染6 h后出现精神萎靡、食欲不振、寒颤、发热、呼吸困难等,感染30 h时最明显;肺组织明显充血、水肿,大量炎性细胞浸润。大鼠PCN感染后6、18、30、48、72 h BALF细胞总数、BALF中性粒细胞计数、ROS含量、SOD活性、IL-8含量比较,差异均有统计学意义(P0.05);其中PCN组和SNAC组各时间点BALF细胞总数、BALF中性粒细胞计数、ROS含量、IL-8含量较对照组均增高,SOD活性较对照组均降低,SNAC组各时间点BALF细胞总数、BALF中性粒细胞计数、ROS含量、IL-8含量较PCN组均降低,SOD活性较PCN组均增高(P0.05)。结论 SNAC对PCN感染鼠体内氧化损伤具有一定的保护作用。
[Abstract]:Objective to investigate the effect of S- nitroso -N- acetylcysteine (SNAC) on the pathomorphology and oxidation of septoin (PCN) infected rats. Methods 90 healthy male SD rats were divided into 3 groups by random number table method, 30 rats in each group were injected with 1 ml/100 g 0.9% Sodium Chloride Solution intraperitoneally in the control group and PCN group, and the abdominal injections of group SNAC rats were intraperitoneally injected. SNAC (1 ml/100 g). Intraperitoneal injection of 1% pentobarbital sodium solution (40 mg/kg), 0.9% Sodium Chloride Solution 0.2 ml in the control group, and PCN 0.2 ml (6 * 108CFU/ml) in group PCN and SNAC group, respectively, and the rats were killed, and the bronchoalveolar lavage fluid and lung were killed in the rats. Tissue, observed the morphological changes of lung tissue, calculated the total number of BALF cells and neutrophils count, detected the content of active oxygen (ROS), the activity of superoxide dismutase (SOD), and the content of interleukin 8 (IL-8). Results after the infection of 6 h, the PCN group was depressed, anorexia, chill, fever, dyspnea and so on. The most obvious infection was 30 h; the lung tissue was obviously filled. The total number of 6,18,30,48,72 h BALF cells, BALF neutrophils count, ROS content, SOD activity and IL-8 content were statistically significant (P0.05) in rats after PCN infection, and the total number of BALF cells in PCN and SNAC groups at all time points, neutrophils count, and content were all compared with the control group. The activity of SOD was lower than that of the control group. The total number of BALF cells at all time points in group SNAC, the count of BALF neutrophils, the content of ROS, the content of IL-8 were all lower than those in the PCN group, and the activity of SOD was higher than that in the PCN group (P0.05). Conclusion SNAC on the oxidative damage in the mice infected with PCN mice has a certain protective effect.
【作者单位】：辽宁医学院附属第一医院呼吸内科;
【基金】：辽宁省自然科学基金资助项目(201102126)
【分类号】：R346

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