脱氧核酶抑制甲型流感病毒复制的体外研究

发布时间：2018-06-02 11:16

本文选题：脱氧核酶 + 流感病毒　；参考：《重庆医科大学》2006年硕士论文

【摘要】： 背景与目的流行性感冒病毒(Influenza virus,IFV),简称流感病毒,是引起流行性感冒的病原体。流感长期以来一直严重威胁着人类的健康,近几年研究发现,禽流感等可以跨越种属差异,由禽类传染人类,给人类的生命、财产安全都带来更大的威胁,引起了全球各界的高度重视。因此,开展流感病毒的研究和防治具有重要的科学意义和实际应用价值。疫苗接种是目前预防病毒性疾病的有效措施。但由于流感病毒表面抗原容易发生变异,致使疫苗预防流感效果欠佳。现在所应用的抗流感药物主要有金刚烷胺类和神经氨酸酶(Neuraminidase,NA)抑制剂类两类药物,由于它们的毒副作用,限制了临床中的应用。因此开发更安全、有效的新型的抗流感病毒药物已势在必行。对流感病毒复制和转录起关键作用的RNA聚合酶(由PB1, PB2和PA三个亚基组成),在流感病毒的生活周期中发挥着重要作用,其中聚合酶碱性蛋白1(PB1)亚基具有RNA聚合酶活性,既参与mRNA转录又参与病毒RNA(vRNA)的复制,并催化新合成的RNA链延伸,变异性相对较小。可以作为一种潜在的抗流感病毒药物的作用靶点,国外已有针对PB1-mRNA反义寡聚核苷酸(ASO)用于抑制病毒复制的研究,其在无细胞系统具有较好的切割效果。
[Abstract]:Background and purpose Influenza virus Influenza virus, or influenza virus, is the causative agent of influenza. Influenza has been a serious threat to human health for a long time. In recent years, studies have found that bird flu and so on can cross species differences, infect humans by birds, and pose a greater threat to human life and property security. It has aroused great attention from all walks of life around the world. Therefore, the research and prevention of influenza virus has important scientific significance and practical application value. Vaccination is an effective measure to prevent viral diseases. However, the surface antigen of influenza virus is easy to mutate, which results in poor effect of vaccine against influenza. The main antiinfluenza drugs used are amantadine and neuraminidase NAA inhibitors, which limit the clinical application due to their side effects. Therefore, the development of safer and more effective new anti-influenza drugs is imperative. The RNA polymerase (composed of PB1, PB2 and PA), which plays a key role in influenza virus replication and transcription, plays an important role in the life cycle of influenza virus. The polymerase basic protein 1 (PB1) subunit has the activity of RNA polymerase. It is involved in both mRNA transcription and viral RNA RNA replication, and catalyzes the newly synthesized RNA strand extension. The variability is relatively small. PB1-mRNA antisense oligodeoxynucleotides (ASO) have been used to inhibit viral replication and have good cleavage effect in acellular system.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R373

【引证文献】