乙醇、乙醛对神经及心脏活动影响的比较研究

发布时间：2018-06-02 15:11

本文选题：乙醇 + 乙醛　；参考：《湖南师范大学》2006年硕士论文

【摘要】：人类过量饮酒导致的问题十分严重,因饮酒而产生的疾病中尤以心脑血管疾病为最。酒精在体内代谢的中间产物为乙醛。以往对神经、心脏影响的研究多侧重于乙醇的影响,国外虽对乙醛的影响有一些研究,但对乙醛、乙醇两者作用的比较研究甚少。针对饮酒对心脑血管的影响,我们选择了以神经和心脏为对象开展乙醇、乙醛作用的比较研究,以探讨两者对器官影响的作用大小及相关机制,为进一步的研究提供基础和参考。将含有不同浓度的乙醇、乙醛的任氏液滴加到蟾蜍坐骨神经表面,30秒后测定神经干刺激阈值、最大刺激值,复合动作电位峰值和不应期等电生理指标。结果表明:乙醇在86～515 mmol/L浓度范围内能使神经标本的不应期缩短,刺激阈值、最大刺激值减小,复合动作电位峰值升高;而乙醛在8.5～42.8 mmol/L浓度范围内即能对标本产生上述影响。如果乙醇浓度提高至859～1545 mmol/L,则神经标本表现出不应期延长,刺激阈值、最大刺激值提高,复合动作电位降低(仍高于空白对照组)等现象;而使神经标本表现出相同现象的乙醛浓度只相应地提升为85.6～429 mmol/L。用高于1.5 mol/L的乙醇处理神经标本,可能对其造成不可恢复性损伤,而乙醛的最低损伤浓度为0.5 mol/L左右。在对心脏的影响研究中,用不同浓度的乙醇和乙醛对蛙心标本进行灌流,用BL-420~+系统对给药前后心脏的心率作记录,以研究乙醇和乙醛对离体心脏和神经干的影响及可能机制。结果显示乙醇对心脏
[Abstract]:The problems caused by excessive drinking in humans are very serious, especially cardiovascular and cerebrovascular diseases. The intermediate product of alcohol metabolism in vivo is acetaldehyde. Previous studies on the effects of nerve and heart focused on the effects of ethanol. Although there are some studies on the effects of acetaldehyde in foreign countries, there are few comparative studies on the effects of acetaldehyde and ethanol. In view of the effects of alcohol drinking on cardiovascular and cerebrovascular diseases, we chose to study the effects of ethanol and acetaldehyde on nerves and hearts in order to explore the effects of alcohol and acetaldehyde on organs and the related mechanisms. To provide the basis and reference for further research. Ren's drops containing different concentrations of ethanol and acetaldehyde were added to the sciatic nerve surface of toad. After 30 seconds, the nerve stem stimulation threshold, the maximum stimulation value, the peak value of complex action potential and the refractory period were measured. The results showed that ethanol could shorten the refractory period, reduce the stimulation threshold, decrease the maximum stimulation value and increase the peak value of compound action potential in the range of 86 ~ 515 mmol/L, while acetaldehyde could produce the above effects in the range of 8.5 ~ 42.8 mmol/L. If ethanol concentration was increased to 859 ~ 1545 mmol / L, the nerve specimens showed prolonged refractory period, increased stimulation threshold, increased maximum stimulation value, and decreased complex action potential (still higher than that of the blank control group). The acetaldehyde concentration, which showed the same phenomenon in the nerve samples, only increased to 85.6 mmol-1 / L at 429 mmol 路L ~ (-1) 路L ~ (-1). The nerve specimen treated with ethanol for more than 1.5 mol/L may cause non-restorative injury, while the lowest injury concentration of acetaldehyde is about 0.5 mol/L. In order to study the effect of ethanol and acetaldehyde on isolated heart and nerve stem, the frog heart was perfused with different concentrations of ethanol and acetaldehyde, and the heart rate before and after administration was recorded by BL-420 ~ system in order to study the effect of ethanol and acetaldehyde on isolated heart and nerve trunk. The results showed that ethanol had effect on the heart.
【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

【参考文献】