碘缺乏和碘过量对大鼠心肌肌球蛋白重链基因mRNA表达的影响

发布时间：2018-06-05 09:26

  本文选题：碘缺乏 + 碘过量　； 参考：《郑州大学》2005年硕士论文

【摘要】：碘是机体合成甲状腺激素(Thyroid Hormone，TH)所必需的微量元素，碘缺乏或碘过量都将影响TH的合成。TH有许多重要的生理功能，心脏是受TH调节的重要靶器官之一，而心肌肌球蛋白重链基因则是TH调节的重要靶基因。肌球蛋白是心肌主要的结构蛋白，也是起收缩作用的主要蛋白。心肌肌球蛋白重链(myosin heavy chain，MHC)包括α和β两条链。然而，碘缺乏和碘过量能否影响MHC基因的表达，，尚未见文献报道。 本实验通过低碘和高碘饲养，造成碘缺乏和碘过量动物模型，观察其对正常大鼠心肌α-MHC和β-MHC mRNA表达的影响。 材料与方法 实验动物：体重120-140g的健康Wistar大鼠90只。将动物随机分为6组：低碘组(LI)，适量碘组(NI)，5倍碘组(5HI)，10倍碘组(10HI)，50倍碘组(50HI)，100倍碘组(100HI)。每组15只大鼠。 LI组动物饲以碘含量小于50μg／kg的低碘饲料，饮用去离子水。NI组和4个HI组大鼠饲以平均碘含量为300μg／kg的正常大鼠饲料；NI组饮用自来水，4个HI组饮用含不同高剂量碘酸钾(KIO_3)的自来水。计算6组大鼠日碘摄入量分别为：＜1μg／d(LI组)、6.15μg／d(NI组)、30.75μg／d(5HI组)、61.5μg／d(10HI组)、307.5μg／d(50HI组)、615μg／d(100HI组)。 大鼠喂养6个月后，称重，抽血测定甲状腺激素，心电图检查，股动脉放血处死动物并迅速摘取心脏，液氮速冻后-80℃保存。 血清甲状腺激素的测定：TT_3、TT_4、FT_3、FT_4采用化学发光法。 心电图描记：LI组由于体格瘦小和麻醉易死未进行心电图检查。NI组和各
[Abstract]:Iodine is an essential trace element in the synthesis of thyroid hormone Thyroid Hormonethra. Iodine deficiency or iodine excess will affect the synthesis of th. Th has many important physiological functions. The heart is one of the important target organs regulated by th. Cardiac myosin heavy chain gene is an important target gene for th regulation. Myosin is the main structural protein of myocardium and the main protein of contraction. Cardiac myosin heavy (MHC) consists of two chains: 伪 and 尾. However, whether iodine deficiency and iodine excess can affect the expression of MHC gene has not been reported. The effects of iodine deficiency and iodine excess on the expression of 伪 -MHC and 尾 -MHC mRNA in myocardium of normal rats were observed. Materials and methods Experimental animals: 90 healthy Wistar rats weighing 120-140 g. The animals were randomly divided into six groups: low iodine group (LI), moderate iodine group (n = 5) with 5 times iodine (n = 5) and 10 times iodine group (n = 10) with 50 times iodine (n = 50) and 100 times iodine group (n = 100). There were 15 rats in each group. The animals of Li group were fed with low iodine diet with iodine content less than 50 渭 g/kg. Rats of deionized water. NI group and 4 HI groups were fed with tap water with average iodine content of 300 渭 g/kg. Four HI groups drank tap water containing different high doses of potassium iodate KIO3). The daily iodine intake was calculated as follows: < 1 渭 g/d(LI group, 6.15 渭 g/d(NI group, 30.75 渭 g/d(5HI group, 61.5 渭 g/d(10HI group, 307.5 渭 g/d(50HI group, 615 渭 g/d(100HI group. After feeding for 6 months, the rats were weighed, blood samples were taken to determine thyroid hormones, electrocardiogram (ECG), femoral artery bloodletting to kill the animals, and the hearts were removed quickly. The rats were preserved at -80 鈩

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