异常黑胆质载体动物模型的建立及其免疫功能的研究

发布时间：2018-06-08 14:05

本文选题：维吾尔医学 + 异常黑胆质　；参考：《新疆医科大学》2006年硕士论文

【摘要】：目的：建立维吾尔医异常黑胆质证载体动物模型，探索异常黑胆质症与免疫功能之间关系。方法：选用ICR小鼠，在阴凉环境下，采用干寒属性饲料饲养、慢性足底电击等多因素复合作用建立维吾尔医异常黑胆质证载体动物模型。观察实验动物行为改变、体重、体温、饮食量、饮水量、尿和大便量，检测有关免疫功能指标。结果：与正常对照组对比，模型组模拟出异常黑胆质证候特征即体形消瘦、情绪焦虑、易怒、攻击性强、倦怠嗜睡、易醒、孤僻、大便干燥、体重增长速度慢(p＜0.001)，饮食量增多(p＜0.001)，饮水量增多(p＜0.001)，尿量增多(p＜0.01)，大便量增多(p＜0.001)。异常黑胆质载体动物模型的免疫功能紊乱，，其中腹腔巨噬细胞吞噬中性红的功能低下(p＜0.001)，豆素(ConA)和脂多糖(LPS)诱导的脾脏淋巴细胞增殖减弱、反应性下降(p＜0.001)，脾脏和外周血淋巴细胞亚群CD4~+百分率降低(p＜0.001)，CD4~+／CD8~+比值倒置(p＜0.001)，胸腺T细胞CD4~+百分率下降(p＜0.05)，CD8~+百分率增高(p＜0.001)，CD4~+／CD8~+比值比值倒置(p＜0.001)。肝脏重量指数高(p＜0.05)，脾脏重量指数低(p＜0.01)，胸腺重量指数低(p＜0.05)。结论：多因素复合作用，能成功地建立维吾尔医异常黑胆质证载体动物模型，其免疫功能是紊乱的。这可能是异常黑胆质证的重要特征。
[Abstract]:Objective: to establish an animal model of abnormal black gallbladder syndrome in Uygur medicine and to explore the relationship between abnormal black gallbladder disease and immune function. Methods: ICR mice were used to establish the animal model of abnormal black gallbladder syndrome of Uygur medicine under the condition of shade and cool, using dry cold attribute feed, chronic plantar electric shock and other multi-factor compound effects. Behavioral changes, body weight, body temperature, diet, drinking water, urine and stool were observed. Results: compared with the normal control group, the model group simulated the characteristics of abnormal black gallbladder syndrome, that is, body shape, emotional anxiety, irritability, aggression, burnout and lethargy, easy to wake up, solitary, dry stool. The growth rate of body weight was less than 0.001g, the amount of diet increased, the quantity of drinking water increased, the quantity of urine increased, and the amount of feces increased (p < 0.001). The immune function of the animal model with abnormal black bilirubin carrier was disturbed, in which peritoneal macrophage phagocytosis of neutral red was decreased (p < 0.001), and the proliferation of splenic lymphocytes induced by lipopolysaccharide (LPSs) and coumarin (Cona) was decreased. The percentage of lymphocyte subsets in spleen and peripheral blood was decreased (p < 0.001), the ratio of CD4 ~ / CD8 ~ was reversed (p < 0.001), the percentage of T cell CD4 ~ was decreased (p < 0.05) and the percentage of CD8 ~ was increased (p < 0.001). The liver weight index, spleen weight index, thymus weight index and thymus weight index were higher (P < 0.05), lower (P < 0.01), and lower (P < 0.05). Conclusion: the animal model of abnormal black gallbladder syndrome carrier of Uygur medicine can be successfully established by multifactor compound action, and its immune function is disordered. This may be an important feature of abnormal black gall examination.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332

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