胰岛发生、发育和再生及相关基因的研究

发布时间：2018-06-10 16:08

本文选题：胰腺发育 + 胰腺干细胞　；参考：《四川大学》2006年博士论文

【摘要】：目的探讨胰腺发育中胰岛细胞的分化、迁移和胰岛的形成，以及在此过程中相关基因表达的变化。对胰岛β细胞损伤后的胰岛再生机制进行初步研究，并检测了β细胞损伤后调控胚胎胰岛细胞分化发育的部分关键性基因Pdx-1、Ngn3、Nkx2.2及Nkx6.1的表达情况，为应用胰腺干细胞作为糖尿病生物治疗性手段提供基础性研究。材料和方法 1．收集征得孕妇签字同意后、因意外等各方面原因必须终止妊娠的不同胎龄段的正常胚胎标本进行组织学观察，并通过免疫组织化学方法观察胰岛细胞的分化发育和CD34、AFP、IGF-I、IGF-IR，TGF-β、TGF-βR，bFGF及FGFR因子在胰腺发育中的表达分布。 2．采用β细胞毒性剂—Alloxan腹腔注射破坏新生SD大鼠的β细胞，复制糖尿病模型，采用免疫组织化学方法观察胰岛β细胞的再生，并通过RT-PCR、Western-blot方法检测Pdx-1、Ngn3、Nkx2.2及Nkx6.1基因的表达。结果 1．人胚胎观察 ① 4周时，，人胚胰芽由前肠末端发出，腹、背胰在胎龄5周时融
[Abstract]:Objective to investigate the differentiation, migration and formation of pancreatic islets during pancreatic development, and the changes of gene expression in the process. The mechanism of islet regeneration after 尾 -cell injury was studied, and the expression of some key genes Pdx-1, Ngn3Ngn3Nkx2.2 and Nkx6.1, which regulated the differentiation and development of embryonic islet cells after 尾 -cell injury, was detected. To provide basic research for the application of pancreatic stem cells as a biotherapeutic method for diabetes mellitus. Materials and methods 1. To collect and obtain the consent of the pregnant woman, to observe the histology of the normal embryo at different stages of gestational age that must be terminated due to accident and other reasons, The differentiation and development of islet cells and the expression of TGF- 尾 TGF- 尾 TGF- 尾 TGF- 尾 and FGFR factor in pancreatic development were observed by immunohistochemical method. 2. 尾 -Alloxan, a cytotoxic agent, was injected intraperitoneally to destroy the 尾 cells of newborn SD rats, and the diabetic model was established. The regeneration of islet 尾 cells was observed by immunohistochemical method. The expression of Nkx2.2 and Nkx6.1 genes was detected by RT-PCRG-Western-blot. Results 1.Results 1. When human embryo was observed for 14 weeks, the bud of human embryo came from the end of the foregut, and the abdomen and dorsal pancreas melted at 5 weeks of gestational age.
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R33

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