肝前型门静脉高压症大鼠模型建立的实验研究

发布时间：2018-06-10 20:50

本文选题：门静脉高压 + 动物模型　；参考：《首都医科大学》2007年硕士论文

【摘要】： 目的本实验旨在通过部分结扎Wistar大鼠的门静脉以建立稳定的肝前型门静脉高压模型,并观测本模型门静脉压力的变化,以利于对肝前型门静脉高压症病理生理及治疗方法进一步研究提供动物模型。材料和方法取30只Wistar雄性大鼠作实验动物,平均体重300g(280-320g)。动物随机分为两组:A组大鼠10只,为假手术组(Sham组),开腹游离门静脉后关腹;B组大鼠20只,为实验组(Portal vein ligation, PVL组),开腹用21G钝针头缩窄门静脉后关腹,结扎前测量门静脉直径和门静脉压力,两组术后2、6、10周测定门静脉压力,观察腹腔内门腔侧枝循环形成情况。结果术后1周内大鼠体重下降,术后2周体重较术前增长。PVL组大鼠结扎术后2周、6周、10周门静脉压力(17.32±1.77cmH2O、17.36±1.93cmH2O、16.82±2.20 cmH2O)与结扎前压力(9.21±0.63cmH2O)比较均有显著性差异。Sham组大鼠术后2周、6周、10周门静脉压力(9.22±0.49cmH2O、9.27±0.43cmH2O、9.21±0.49cmH2O)与PVL组大鼠同期压力比较有显著性差异。本次实验大鼠门静脉缩窄率约为88.9%。实验中发现大鼠腹腔出现了大量的门腔静脉间侧枝循环,包括腹膜后门腔静脉间的静脉丛、左肾上腺静脉明显扩张并肉眼可见与门静脉所属的曲张静脉丛相连等。结论用21G钝针和4号丝线对Wistar大鼠行门静脉部分结扎术,可以得到满意的肝前型门静脉高压Wistar大鼠模型,本实验结果可以作为制作肝前型门静脉高压症Wistar大鼠模型的标准。
[Abstract]:Objective to establish a stable prehepatic portal hypertension model by partial ligation of portal vein in Wistar rats and observe the changes of portal vein pressure in this model. Materials and methods 30 male Wistar rats were used as experimental animals with an average body weight of 300g / 320g / kg. The animals were randomly divided into two groups: sham group (n = 10), sham group (n = 20), portal vein ligation group (n = 20) and portal vein ligation group (n = 20). The diameter and pressure of portal vein were measured before ligation, and the portal vein pressure was measured 10 weeks after operation, and the formation of collateral circulation in abdominal cavity was observed. Results the weight of rats decreased within 1 week after operation. There were significant differences in body weight 2 weeks after operation compared with those before ligation. The portal vein pressure was 17.32 卤1.77 cm H 2O 17.36 卤1.93 cm H 2O 16.82 卤2.20 cm H 2O at 2 weeks after ligation and 9.21 卤0.63 cm H 2O before ligation. The portal vein pressure was 9.22 卤0.49 cm H 2O 9.22 卤0.49 cm H 2O 9.27 卤0.43 cm H 2O 9.27 卤0.49 cm H 2O 9.27 卤0.43 cm H 2O 9.21 卤0.49 cm H 2O in the sham group. There was a significant difference in the pressure in the same period. The coarctation rate of portal vein in this experiment was about 88. 9%. It was found that a large number of portal vena cava collateral circulation appeared in the abdominal cavity of rats, including the venous plexus between the retroperitoneal vena cava. The left adrenal vein was obviously dilated and connected with the variceal plexus attached to the portal vein with the naked eye. Conclusion the partial ligation of portal vein was performed on Wistar rats with 21G blunt needle and 4 ~ (th) silk thread. A satisfactory model of prehepatic portal hypertension in Wistar rats can be obtained. The results of this experiment can be used as the standard for making Wistar rat model of prehepatic portal hypertension.
【学位授予单位】：首都医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332

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