TGF-β1联合TNBS诱导Crohn病肠纤维化模型

发布时间：2018-06-13 23:27

本文选题：Crohn病 + 肠纤维化　；参考：《中国普通外科杂志》2014年09期

【摘要】：目的:观察转化生长因子β1(TGF-β1)联合2,4,6-三硝基苯磺酸(TNBS)诱导大鼠Crohn病(CD)肠纤维化模型的效果。方法:将SD大鼠30只随机分为模型组(20只)和对照组(10只)。模型组大鼠予距肛门8 cm处注入1 mL含有TNBS及99%乙醇,24 h后于距肛门4 cm处灌入500μL含携带TGF-β1的腺病毒载体(1×109 pfu/mL滴度)的PBS;对照组大鼠不做处理。观察大鼠造模后的一般情况、行活动度评分(DAI),造模后12 d处死大鼠,取大鼠结肠组织,行大体病理与组织病理学观察,并评分。结果:模型组大鼠于造模后第2天,出现腹泻、血便、脓血便等症状,第4天达到高峰,然后渐渐好转,对照组大鼠精神状态、进食及活动如常;模型组第4、8、12天的DAI值明显高于对照组(均P0.05);模型组大鼠体质量在诱导后第4~5天明显下降,此后体质量缓慢增加,对照组大鼠的体质量则持续增加,实验结束时模型组大鼠的体质量增幅明显小于对照组(P0.05)。模型组结肠明显充血水肿、溃疡形成,镜下可见明显的纤维化病变,对照组结肠未见明显异常,模型组的大体病理评分及组织病理评分均明显高于对照组(均P0.05)。结论:TGF-β1联合TNBS制作大鼠CD肠纤维化模型,方法简单、重复性好、模拟效果佳。
[Abstract]:Aim: to observe the effect of transforming growth factor 尾 1 TGF- 尾 1 (TGF- 尾 1) combined with 2 trinitrobenzene 6 trinitrobenzene sulfonic acid (TNBS) on the model of Crohn's disease (CD) in rats. Methods: thirty SD rats were randomly divided into model group (n = 20) and control group (n = 10). Rats in the model group were injected with 1 mL containing TNBS and 99% ethanol for 24 h at 8 cm from the anus, and 500 渭 L containing TGF- 尾 1 adenovirus vector containing TGF- 尾 1 was injected into the anus for 24 h, but the control group was not treated. The general situation of the rats was observed. The rats were killed 12 days after the establishment of the model. The colonic tissues of the rats were taken out and the gross and histopathological observations were made. Results: the rats in the model group had symptoms of diarrhea, blood stool, pus and blood stool on the second day after the establishment of the model, and reached the peak on the 4th day, then gradually improved. In the control group, the mental state, food intake and activity of the rats were normal. The Dai value of the model group was significantly higher than that of the control group on the 12th day (all P 0.05), the body mass of the model group decreased significantly on the 4th day after induction, and then the body mass increased slowly, while the body mass of the control group increased continuously. At the end of the experiment, the increase of body mass in the model group was significantly lower than that in the control group (P 0.05). There were obvious hyperemia and edema, ulceration and fibrosis under microscope in the model group, but no obvious abnormality in the colon in the control group. The gross and histopathological scores in the model group were significantly higher than those in the control group (all P 0.05). Conclusion the rat model of CD intestinal fibrosis made by TGF- 尾 1 and TNBS is simple, reproducible and simulated.
【作者单位】：东南大学附属中大医院普通外科;
【基金】：国家自然科学基金资助项目(81000153) 江苏省自然科学基金资助项目(BK2010415)
【分类号】：R-332;R574

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