2007～2012年山东省临沂市手足口病流行病学和肠道病毒71型基因特征分析

发布时间：2018-06-14 10:52

本文选题：手足口病 + 流行病学　；参考：《病毒学报》2014年03期

【摘要】：为了分析山东省临沂市2007~2012年手足口病(Hand,foot,and mouth disease,HFMD)的流行病学和肠道病毒71型(Enterovirus A71,EV-A71)的基因特征,采用描述流行病学方法,对国家传染病监测信息报告管理系统中的HFMD疫情资料进行统计分析;对2007~2012年山东省临沂市HFMD病例的EV-A71分离株的VP1编码区进行逆转录-聚合酶链反应,并对扩增产物进行核苷酸序列测定,使用MEGA 5.0软件分析VP1区基因变异特征以及基因亲缘关系。结果显示,2007~2012年山东临沂市每年均有HFMD报告,其中2009年发病例数和死亡病例数最多,分别为14 697例和9例,报告发病率为140.28/10万,死亡率为0.086/10万。发病高峰主要发生在4~7月,5月份最高;其中散居儿童发病数占77.37%~92.00%。2007~2009年发病高峰在2.5岁,2010~2012年发病高峰在1.5岁。连续6年实验室确诊HFMD共计1 365例,占总病例数比例为2.98%;其中EV-A71构成比为44.18%,柯萨奇病毒A组16型(Coxsackievirus A16,CVA16)为46.59%。2007~2012年在山东省临沂市分离到的EV-A71均属于C4基因亚型中C4a进化分支。2007~2012年山东省临沂市均有HFMD暴发流行,各区县发病率有明显差异,发病高峰在每年的4~7月。发病年龄集中在3岁以下儿童,散居儿童构成比例最高。各年份引起HFMD的病原体均以EV-A71和CVA16共循环为主。自2007年在山东省临沂市发现EV-A71C4基因亚型的C4a引起的HFMD疫情以来,C4a在临沂市已经持续传播和流行了6年。
[Abstract]:In order to analyze the epidemiology of hand foot and mouth disease (HFMDD) and the gene characteristics of Enterovirus A71 (EV-A71) from 2007 to 2012 in Linyi City, Shandong Province, a descriptive epidemiological method was used. The epidemic data of HFMD in the national infectious disease surveillance information management system were statistically analyzed, and the VP1 coding region of EV-A71 isolated strains of HFMD cases from 2007 to 2012 in Linyi City, Shandong Province, was analyzed by reverse transcription-polymerase chain reaction (RT-PCR). The nucleotide sequence of the amplified product was determined and the variation characteristics and genetic relationship of VP1 region were analyzed by MEGA5.0 software. The results showed that HFMD was reported every year in Linyi City, Shandong Province from 2007 to 2012, in which 14697 cases and 9 deaths were reported in 2009, respectively. The reported incidence rate was 140.28% / 100 000, and the mortality rate was 0.086% / 100 000. The incidence peak occurred mainly in April ~ July and May, and 77.37% of which occurred in scattered children. The peak of disease occurred from 2.5 years old to 2010 ~ 2012 at 1.5 years old. A total of 1 365 cases of HFMD were confirmed in the laboratory for 6 consecutive years. The proportion of EV-A71 to total cases was 2.98, in which the constituent ratio of EV-A71 was 44.18, Coxsackievirus A16 of Coxsackievirus A was 46.59.2007. EV-A71 isolated from Linyi City, Shandong Province, from 2007 to 2012, all belonged to C4a evolutionary branch of C4 gene subtype. From 2007 to 2012, there were HFMD outbreaks in Linyi City, Shandong Province. There were significant differences in the incidence of disease among districts and counties, and the peak of incidence was from April to July of each year. The onset age was concentrated in children under 3 years of age, with the highest proportion of children living in the diaspora. The common circulation of EV-A71 and CVA16 were the main pathogens of HFMD in each year. Since the HFMD epidemic caused by EV-A71C4 gene subtype C4a was discovered in Linyi City of Shandong Province in 2007, C4a has been continuously spreading and epidemic in Linyi City for 6 years.
【作者单位】：中国疾病预防控制中心病毒病预防控制所世界卫生组织西太平洋区脊髓灰质炎参比实验室;山东省疾病预防控制中心;
【基金】：973(项目号2011CB504902) 国家科技重大专项(项目号。2012ZX10004201-003;2013ZX10004202) 山东省科学技术发展计划(项目号2009GG10002055) 山东省自然科学基金(项目号:ZR2013HM052)
【分类号】：R373.9

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