细胞凋亡和细胞自噬在慢性环孢素A肾毒性中的作用

发布时间：2018-06-16 08:04

  本文选题：慢性CsA肾毒性 + 细胞凋亡　； 参考：《中国老年学杂志》2014年11期

【摘要】：目的探讨细胞凋亡和细胞自噬在慢性环孢素A(CsA)肾毒性中的作用。方法 Sprague-Dawley大鼠分为两组:对照组:皮下注射橄榄油(1 ml·kg-1·d-1)4 w;慢性CsA肾毒性组:皮下注射CsA(15 mg·kg-1·d-1)4 w。检测两组大鼠的体重和肾功能;三色染色检测肾小管间质纤维化程度;ELISA法检测血、尿8羟基脱氧鸟苷(8-OHdG)水平;原位末端标记法(TUNEL)染色观察细胞凋亡;免疫印迹法检测细胞凋亡调控基因(Bcl-2、Bax、Caspase-3)和细胞自噬体膜型LC3-Ⅱ蛋白的表达;Pearson直线相关分析肾小管间质纤维化程度与细胞凋亡和LC3-Ⅱ蛋白表达的相关关系。结果与对照组相比,毒性组大鼠体重减轻、肾功能低下、血尿8-OHdG水平升高,同时肾毒性组可见明显的肾小管间质纤维化和大量TUNEL阳性细胞。免疫印迹结果表明,毒性组Bax、Caspase-3、LC3-Ⅱ蛋白的表达明显增加,反之,Bcl-2的表达显著减少。直线相关分析提示,肾小管间质纤维化程度与TUNEL阳性细胞数和LC3-Ⅱ蛋白表达呈正向相关。结论细胞凋亡和细胞自噬参与了慢性CsA肾毒性肾小管间质的损伤。
[Abstract]:Objective to investigate the role of apoptosis and autophagy in chronic cyclosporine (CSA) nephrotoxicity. Methods Sprague-Dawley rats were divided into two groups: the control group was subcutaneously injected with 1 ml kg-1 d ~ (-1) of olive oil for 4 weeks, and the chronic CSA nephrotoxicity group was subcutaneously injected with CSA 15 mg kg-1 d ~ (-1) for 4 weeks. The weight and renal function of the rats in the two groups were measured, the degree of renal tubulointerstitial fibrosis was detected by trichromatic staining and the levels of blood and urine 8-OHdG were detected by Elisa, apoptosis was observed by in situ end labeling and Tunel staining. Expression of apoptosis-regulating gene (Bcl-2) and macrophage membrane type LC3- 鈪，

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