结核分枝杆菌感染小鼠模型的建立与评价

发布时间：2018-08-03 13:34

【摘要】：目的建立结核分枝杆菌感染的小鼠模型,分析感染小鼠重量指数、组织荷菌量、组织病理改变随感染时间的变化。方法以1.1×105菌落形成单位(CFU)的结核分枝杆菌标准株H37Rv经尾静脉感染50只雌性BALB/c小鼠,于感染后1、2、3、4、8周杀鼠,每个时间点剖杀10只,观察肺组织病理改变并称重,计算重量指数,同时做肺和肝菌落计数。结果感染1、2、3、4、8周小鼠的肺脏器重量指数分别为0.0112±0.0036、0.0101±0.0071、0.0112±0.0046、0.0151±0.0057和0.0198±0.0069,差异有统计学意义(F=4.24,P0.01);肝脏器重量指数分别为0.0558±0.0059、0.0591±0.0094、0.0569±0.0076、0.0582±0.0030和0.0619±0.0079,差异无统计学意义(F=0.86,P0.05);脾脏器重量指数分别为0.0107±0.0034、0.0146±0.0060、0.018±0.0034、0.0174±0.0026和0.0204±0.0066,差异有统计学意义(F=4.01,P0.05);肺菌落数分别为(4.472±0.504)log、(5.539±0.429)log、(6.294±0.478)log、(6.250±0.315)log和(6.836±0.196)log,差异有统计学意义(F=43.90,P0.01)。感染后1周小鼠肺组织可见病理改变,且随着感染时间的增加病变范围逐渐扩大,病变程度逐渐严重。结论成功建立了结核分枝杆菌感染小鼠模型,该模型可用于结核疫苗和药物研究。
[Abstract]:Objective to establish a mouse model of Mycobacterium tuberculosis infection, to analyze the weight index, tissue load and histopathological changes of infected mice with the infection time. Methods 50 female BALB/c mice were infected with 1.1 x 105 colony forming unit (CFU) H37Rv by tail vein infection, and mice were killed at 1,2,3,4,8 weeks after infection, each time after infection. The pathological changes and weight of lung tissue were observed and weighed, and the weight index was calculated, and the lung and liver colonies were counted at the same time. The lung organ weight index of the mice infected with 1,2,3,4,8 weeks was 0.0112 + 0.0036,0.0101 + 0.0071,0.0112 + 0.0046,0.0151 + 0.0057 and 0.0198 + 0.0069, respectively, and the difference was statistically significant (F=4.24, P0.01), and the weight of liver organs. The indexes were 0.0558 + 0.0059,0.0591 + 0.0094,0.0569 + 0.0076,0.0582 + 0.0030 and 0.0619 + 0.0079 respectively, the difference was not statistically significant (F=0.86, P0.05), and the weight index of spleen was 0.0107 + 0.0034,0.0146 + 0.0034,0.0174 + 0.0026 and 0.0204 + 0.0066 respectively. The difference was statistically significant (F=4.01, P0.05), and the number of pulmonary colonies was 4 (4). .472 + 0.504) log, (5.539 + 0.429) log, (6.294 + 0.478) log, (6.250 + 0.315) log and (6.836 + 0.196) log, the difference was statistically significant (F=43.90, P0.01). The pathological changes in lung tissues of mice were seen in 1 weeks after infection, and the extent of pathological changes gradually increased with the increase of infection time. Conclusion the sense of Mycobacterium tuberculosis was successfully established. The mouse model can be used for tuberculosis vaccine and drug research.
【作者单位】：解放军第309医院全军结核病研究所军队结核病防治重点实验室;
【基金】：国家重大传染病专项资助项目(No.2008ZX1000301302,2012ZX10003008002)
【分类号】：R378;R-332

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