前列腺增生大鼠模型的建立

发布时间：2018-08-08 22:01

【摘要】：目的:探讨丙酸睾酮对去势和不去势SD大鼠前列腺增生的影响。方法:8周龄雄性SD大鼠25只,随机分为正常对照组(n=5)、不去势模型组(n=15)及去势模型组(n=5);不去势模型组按注射丙酸睾酮剂量又分为不去势低剂量组、中剂量组和高剂量组,注射剂量分别为1.25,2.5,5 mg/(kg·d);去势模型组无菌切除双侧睾丸1周后注射丙酸睾酮5 mg/(kg·d);去势模型组和不去势模型组均连续注射丙酸睾酮4周,于末次给药后分离大鼠前列腺,检测其湿重、体积和脏器系数;组织切片HE染色,光镜下观察前列腺组织变化。结果:不去势小剂量组和中剂量组的前列腺湿重、体积和脏器系数增加,与正常对照组相比差异有统计学意义(P0.05),不去势高剂量组和去势组的前列腺体积和脏器系数与正常对照组相比,差异均具有统计学意义(P0.01);去势组和不去势组大鼠前列腺体积和腺腔大小均较正常对照组增大,且不去势组前列腺腺体与周围组织界限更清楚,腺腔内乳头状突起也比去势组更加明显。结论:使用不去势、皮下注射5 mg/(kg·d)丙酸睾酮的方法可有效的建立前列腺增生大鼠模型。
[Abstract]:Aim: to investigate the effect of testosterone propionate on prostatic hyperplasia in ovariectomized and non-castrated SD rats. Methods Twenty-five male Sprague-Dawley rats aged 8 weeks were randomly divided into normal control group (n = 5), no castration model group (n = 15) and ovariectomized model group (n = 5), and no castration model group was divided into no castration low dose group, middle dose group and high dose group according to the dose of testosterone propionate injection. The rats in ovariectomized group were injected with testosterone propionate 5 mg/ (kg d); for 1 week, and testosterone propionate was injected continuously for 4 weeks in the ovariectomized model group and the non-castrated model group. The prostatic gland was separated after the last administration and its wet weight was measured. Volume and organ coefficient, HE staining of tissue sections, changes of prostate tissue under light microscope. Results: the wet weight, volume and organ coefficient of prostate were increased in the low dose group and middle dose group. Compared with the normal control group, the prostate volume and organ coefficient of the non-ovariectomized high dose group and ovariectomized group were significantly higher than that of the normal control group (P0.05). The difference was statistically significant (P0.01), the volume of prostate and the size of glandular cavity in ovariectomized group and non-castrated group were larger than those in normal control group, and the boundary between prostate gland and surrounding tissue in non-castrated group was more clear than that in non-castrated group. The intraglandular papillary process was also more pronounced than that in the castrated group. Conclusion: subcutaneous injection of testosterone propionate for 5 mg/ (kg d) can effectively establish a rat model of benign prostatic hyperplasia (BPH).
【作者单位】：宁夏医科大学检验学院;宁夏医科大学实验动物中心;
【基金】：国家自然科学基金(81201344) 宁夏医科大学面上项目(XM2011008)
【分类号】：R697.32;R-332

【相似文献】