糖尿病小鼠接种禽流感病毒灭活疫苗的免疫应答及保护作用研究

发布时间：2018-08-10 07:35

【摘要】：禽流感是由A型流感病毒引起的禽类急性传染病。目前,禽流感在全世界范围内广泛流行,每一次严重的爆发都给世界经济造成巨大的损失。 1997年,高致病性禽流感病毒H5N1首次跨种属感染人类,造成香港地区18人感染禽流感,其中6人死亡。随后不断有国家爆发H5N1禽流感疫情,引起多人感染,并伴有相当高的死亡率。1999年,首次发现禽流感H9N2病毒感染香港两名儿童,之后在香港和广东地区人类身上也分离到该亚型病毒。这说明禽流感病毒已具有直接感染人类的能力。糖尿病患者由于全身代谢紊乱,免疫力低下,容易受病毒性疾病的感染,极有可能成为流感流行的最先受害者和疾病的传播者。接种疫苗是糖尿病病人预防病毒性传染病的重要手段。鉴于禽流感对糖尿病患者的生命健康的危害,我们应当致力于针对糖尿病病人的禽流感疫苗的研制,以期为这些免疫力低下的特殊人群提供有效的保护。本论文主要是研究糖尿病小鼠接种禽流感H9N2病毒灭活疫苗的免疫应答及其保护作用。将A/chicken/Jiangsu/07/2002(H9N2)病毒经肺对肺传代,建立BALB/c小鼠适应株,制备禽流感全病毒灭活疫苗。用一次性大剂量STZ腹腔注射的方法,建立糖尿病小鼠模型。分别用不同剂量的灭活疫苗免疫健康小鼠和糖尿病小鼠一次或两次。用致死量禽流感病毒攻击后,用ELISA的方法测定血清中特异性抗体
[Abstract]:Avian influenza is an acute avian infectious disease caused by influenza A virus. At present, bird flu is widespread in the world, and every serious outbreak has caused enormous losses to the world economy. In 1997, The highly pathogenic avian influenza virus (H5N1) is the first to infect humans across species, causing 18 people in Hong Kong to become infected with bird flu, six of them dead. Subsequently, a series of H5N1 bird flu outbreaks occurred in various countries, causing many people to become infected and with a relatively high mortality rate. In 1999, it was first discovered that the avian influenza H9N2 virus was infected with two children in Hong Kong. The subtype was subsequently isolated from humans in Hong Kong and Guangdong. This shows that the avian influenza virus has the ability to infect humans directly. Due to the disorder of systemic metabolism and low immunity, diabetic patients are susceptible to viral diseases, and may become the first victims and the disseminators of influenza. Vaccination is an important means to prevent viral infectious diseases in diabetic patients. In view of the harm of avian influenza to the life and health of diabetic patients, we should devote ourselves to the development of avian influenza vaccine for diabetic patients in order to provide effective protection for these special people with low immunity. In this paper, the immune response and protective effect of inactivated vaccine against avian influenza H9N2 virus in diabetic mice were studied. The A/chicken/Jiangsu/07/2002 (H9N2) virus was passed through the lung to establish the adaptive strain of BALB/c mice to prepare the inactivated vaccine of Avian Influenza virus (Avian Influenza). The model of diabetic mice was established by one-time large-dose STZ intraperitoneal injection. Healthy mice and diabetic mice were immunized with different doses of inactivated vaccine once or twice. The specific antibody in serum was determined by ELISA after the lethal dose of avian influenza virus was attacked.
【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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