C3与迟发型超敏反应及C3和MBL对巨噬细胞吞噬活性的影响
发布时间：2018-08-11 10:41
【摘要】：补体系统是天然免疫的重要组成部分,由30余种可溶性蛋白和膜结合蛋白组成,具有调理和溶解微生物、趋化和过敏毒素、清除免疫复合物等生物学功能。补体成分3(complement component 3,C3)是补体系统的中枢,补体激活的3条途径即经典途径、旁路途径和凝集素途径都要通过激活C3才得以实现。C3可能是补体系统中功能最多的分子,它能与20余种蛋白质相互作用,产生多种功能。近年来在C3基因敲除(C34~(-/-))小鼠的研究提示,C3可以触发和调节获得性免疫,形成了天然免疫和获得性免疫之间的桥梁。然而,C3在细胞免疫或DTH反应中的作用究竟如何,作用有多大,还不很清楚。 甘露聚糖结合凝集素(mannan-binding lectin,MBL)系C型凝集素超级家族中胶凝素家族成员,是天然免疫系统中的关键分子。它可选择性识别多种病原体表面的糖结构,以不依赖抗体和C1q的方式激活补体系统,发挥溶破和间接调理功能,还能与吞噬细胞表面胶凝素受体结合而起直接调理作用。但是,在调理吞噬中,补体系统的作用、MBL的直接调理作用究竟有多大?目前尚无明确的结论。 因此,本研究用卵白蛋白(ovalbumin,OVA)在C3基因敲除小鼠及野生型小鼠诱导足垫的DTH反应,探索C3在DTH中的可能作用;分别用C3~(-/-)小鼠和C3~(+/+)小鼠的血清处理各种病原体,并用抗MBL多克隆抗体处理血清,探索C3和MBL对巨噬细胞体外吞噬功能的影响。 一、目的 1.在C3~(-/-)和C3~(+/+)小鼠(C57BL/6)诱导DTH,探索C3在DTH中的作用。 2.研究MBL体外结合微生物的能力,探索C3和MBL对巨噬细胞吞噬功能的影响。
[Abstract]:Complement system is an important part of innate immunity. It is composed of more than 30 kinds of soluble proteins and membrane binding proteins. It has the biological functions of regulating and dissolving microorganisms chemotaxis and allergy toxins clearing immune complexes and so on. Complement component 3 (C 3) is the center of the complement system. The three pathways of complement activation are classical pathways. Both the bypass pathway and lectin pathway can be realized by activating C3. C3 may be the most functional molecule in the complement system. It can interact with more than 20 proteins and produce many functions. In recent years, studies in C34- / (- / -) mice have suggested that C3 can trigger and regulate acquired immunity and form a bridge between innate immunity and acquired immunity. However, the role and extent of C 3 in cellular immunity or DTH response is unclear. Mannan binding lectin (mannan-binding), a member of the C type lectin superfamily, is a key molecule in the innate immune system. It can selectively recognize the sugar structure on the surface of many pathogens, activate the complement system in a manner independent of antibodies and C1q, play the functions of dissolution and indirect conditioning, and play a direct role in regulating the surface of phagocytes by binding to the gelatin receptor. However, in conditioning and phagocytosis, the role of complement system in the direct conditioning of MBL is how much? There is no clear conclusion. Therefore, ovalbumin OVA was used to induce the DTH response of foot pad in C3 knockout mice and wild-type mice, to explore the possible role of C3 in DTH, and to treat various pathogens with the serum of C3- /-and C3- / mice, respectively. To explore the effect of C3 and MBL on phagocytosis of macrophages in vitro. First, objective 1. DTHs were induced in C3- /-and C3- / mice (C57BL/6) to explore the role of C3 in DTH. 2. To explore the effect of C3 and MBL on phagocytosis of macrophages by studying the ability of MBL to bind microorganism in vitro.
【学位授予单位】：第一军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392
本文编号：2176766
[Abstract]:Complement system is an important part of innate immunity. It is composed of more than 30 kinds of soluble proteins and membrane binding proteins. It has the biological functions of regulating and dissolving microorganisms chemotaxis and allergy toxins clearing immune complexes and so on. Complement component 3 (C 3) is the center of the complement system. The three pathways of complement activation are classical pathways. Both the bypass pathway and lectin pathway can be realized by activating C3. C3 may be the most functional molecule in the complement system. It can interact with more than 20 proteins and produce many functions. In recent years, studies in C34- / (- / -) mice have suggested that C3 can trigger and regulate acquired immunity and form a bridge between innate immunity and acquired immunity. However, the role and extent of C 3 in cellular immunity or DTH response is unclear. Mannan binding lectin (mannan-binding), a member of the C type lectin superfamily, is a key molecule in the innate immune system. It can selectively recognize the sugar structure on the surface of many pathogens, activate the complement system in a manner independent of antibodies and C1q, play the functions of dissolution and indirect conditioning, and play a direct role in regulating the surface of phagocytes by binding to the gelatin receptor. However, in conditioning and phagocytosis, the role of complement system in the direct conditioning of MBL is how much? There is no clear conclusion. Therefore, ovalbumin OVA was used to induce the DTH response of foot pad in C3 knockout mice and wild-type mice, to explore the possible role of C3 in DTH, and to treat various pathogens with the serum of C3- /-and C3- / mice, respectively. To explore the effect of C3 and MBL on phagocytosis of macrophages in vitro. First, objective 1. DTHs were induced in C3- /-and C3- / mice (C57BL/6) to explore the role of C3 in DTH. 2. To explore the effect of C3 and MBL on phagocytosis of macrophages by studying the ability of MBL to bind microorganism in vitro.
【学位授予单位】：第一军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392
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