正常人表皮神经形态计量学研究
发布时间：2018-08-15 11:36
【摘要】： 目的:通过皮肤神经活检,对正常人的表皮内神经纤维(IENFs)进行形态学观察,并对其进行定量研究,测定表皮神经纤维密度(IENFD)。比较年龄、解剖部位以及种族对表皮神经分布的影响,探讨正常人小腿远端IENFD参考值范围。为皮肤神经活检在周围神经病诊断、病程监测以及预后判定等方面的基础研究及临床应用奠定基础。 方法:对选自外科手术的71例正常皮肤标本(其中包括手指指腹皮肤标本1例)进行皮肤神经活检。标本经10%福尔马林常规固定后切为10μm的石蜡切片,以PGP9.5为特异性轴突标记物进行免疫组织化学染色。观察皮肤神经纤维的形态学特点,并测定IENFD,进行统计学分析。 结果:1.皮肤神经以及触觉小体对抗PGP9.5免疫反应性好,IENFs在表皮内多垂直于表皮走形,走行过程中可有分支现象。2.小腿远端各年龄组间IENFD比较差异无显著性,P=0.4780.05;腕部各年龄组间IENFD比较差异无显著性,P=0.8300.05。3.各解剖部位间IENFD比较差异有显著性,P=0.0010.05。腕部组与上臂组及大腿组比较,差异均有显著性(P=0.0010.05, P=0.0060.05),腕部IENFD较上臂及大腿低;小腿组与上臂组及大腿组比较,差异均有显著性(P=0.0010.05,P=0.0050.05),小腿IENFD较上臂及大腿低。小腿远端IENFD为62.87±15.25 fibers/mm~2,其正常参考值范围为40.55 fibers/mm~2。4.我国正常人群小腿远端IENFD低于芬兰人(p0.05)。 结论:1.皮肤活检测定IENFD取材方便、操作简单、费用低廉、有量化的观察指标,故有很大临床应用价值;2.正常人IENFs越过真表皮交界处后,向上垂直走形,根据分支形态可分为四种类型。3.国人小腿远端IENFD平均值为62.87±15.25 fibers/mm~2,正常值参考范围为40.55 fibers/mm~2; 4.年龄对小腿远端和腕部的IENFD无明显影响,不同解剖部位IENFD不同,肢体近端部位IENFD较远端高;5.种族可能对IENFD有影响,我国人群小腿远端IENFD较芬兰人低。
[Abstract]:Objective: to observe the morphology of intraepidermal nerve fiber (IENFs) in normal human by skin nerve biopsy, and to measure the density of epidermal nerve fiber (IENFD).) by quantitative study. To compare the influence of age, anatomical location and race on the distribution of epidermal nerve, and to explore the range of IENFD reference value of distal leg in normal subjects. To lay a foundation for the basic research and clinical application of skin nerve biopsy in the diagnosis, course monitoring and prognosis determination of peripheral neuropathy. Methods: skin nerve biopsy was performed in 71 normal skin samples (including 1 finger skin specimen). The specimens were fixed with 10% formalin and then cut into 10 渭 m paraffin sections. PGP9.5 was used as the specific axon marker for immunohistochemical staining. The morphological characteristics of skin nerve fibers were observed, and the IENFDs were measured for statistical analysis. The result is 1: 1. Skin nerves and tactile corpuscles counteract the immunoreactivity of PGP9.5. Most of them are perpendicular to the epidermis in the epidermis, and the branching phenomenon can occur in the course of walking. There was no significant difference in IENFD between different age groups of distal leg, and there was no significant difference in IENFD between different age groups in wrist. There was a significant difference in IENFD between different anatomical sites (P < 0. 0010.05). There were significant differences between the wrist group and the upper arm group and the thigh group (P0. 0010.05, P0. 006 0. 05). The IENFD of the wrist was lower than that of the upper arm and thigh, and that of the leg group was significantly lower than that of the upper arm and thigh group (P 0. 0010. 05). The IENFD of the lower leg was lower than that of the upper arm and thigh. The IENFD of distal leg was 62.87 卤15.25 fibers / mm-2, and its normal reference value was 40.55 fibers / mm-1 / mm-2. The IENFD of distal leg in normal population was lower than that in Finns (p0. 05). Conclusion 1. The measurement of IENFD by skin biopsy is convenient, easy to operate, low cost and has quantitative observation index, so it has great clinical application value. After crossing the junction of the true epidermis, the normal human IENFs can be divided into four types. 3. The average IENFD of distal leg in Chinese is 62.87 卤15.25 fibers / mmm-2, and the normal reference range is 40.55 fibers / mmm-2; 4. Age had no significant effect on IENFD of distal leg and wrist. IENFD was different in different anatomical sites, and IENFD in proximal extremity was 5% higher than that in distal extremity. Race may have an effect on IENFD and IENFD of distal leg in Chinese is lower than that in Finn.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R33
本文编号：2184082
[Abstract]:Objective: to observe the morphology of intraepidermal nerve fiber (IENFs) in normal human by skin nerve biopsy, and to measure the density of epidermal nerve fiber (IENFD).) by quantitative study. To compare the influence of age, anatomical location and race on the distribution of epidermal nerve, and to explore the range of IENFD reference value of distal leg in normal subjects. To lay a foundation for the basic research and clinical application of skin nerve biopsy in the diagnosis, course monitoring and prognosis determination of peripheral neuropathy. Methods: skin nerve biopsy was performed in 71 normal skin samples (including 1 finger skin specimen). The specimens were fixed with 10% formalin and then cut into 10 渭 m paraffin sections. PGP9.5 was used as the specific axon marker for immunohistochemical staining. The morphological characteristics of skin nerve fibers were observed, and the IENFDs were measured for statistical analysis. The result is 1: 1. Skin nerves and tactile corpuscles counteract the immunoreactivity of PGP9.5. Most of them are perpendicular to the epidermis in the epidermis, and the branching phenomenon can occur in the course of walking. There was no significant difference in IENFD between different age groups of distal leg, and there was no significant difference in IENFD between different age groups in wrist. There was a significant difference in IENFD between different anatomical sites (P < 0. 0010.05). There were significant differences between the wrist group and the upper arm group and the thigh group (P0. 0010.05, P0. 006 0. 05). The IENFD of the wrist was lower than that of the upper arm and thigh, and that of the leg group was significantly lower than that of the upper arm and thigh group (P 0. 0010. 05). The IENFD of the lower leg was lower than that of the upper arm and thigh. The IENFD of distal leg was 62.87 卤15.25 fibers / mm-2, and its normal reference value was 40.55 fibers / mm-1 / mm-2. The IENFD of distal leg in normal population was lower than that in Finns (p0. 05). Conclusion 1. The measurement of IENFD by skin biopsy is convenient, easy to operate, low cost and has quantitative observation index, so it has great clinical application value. After crossing the junction of the true epidermis, the normal human IENFs can be divided into four types. 3. The average IENFD of distal leg in Chinese is 62.87 卤15.25 fibers / mmm-2, and the normal reference range is 40.55 fibers / mmm-2; 4. Age had no significant effect on IENFD of distal leg and wrist. IENFD was different in different anatomical sites, and IENFD in proximal extremity was 5% higher than that in distal extremity. Race may have an effect on IENFD and IENFD of distal leg in Chinese is lower than that in Finn.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R33
【参考文献】
相关期刊论文 前1条
1 周晓伟,魏保庆,包国祥,于基政,沈建平;成年男尸的皮神经分布[J];南京军医学院学报;2000年01期
，本文编号：2184082
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