大鼠非人工呼吸机下急性心肌梗死模型的研究

发布时间：2018-08-17 19:15

【摘要】：心血管疾病的研究少不了动物模型的参与。利用动物模型来模仿人类所患心血管疾病,不仅可以避免人体试验的风险,而且可以随时复制疾病的模型,这样有利于开展研究,使得我们可以从本质上研究这种疾病,探讨疾病的发病机制,并且找出合适的治疗方法[1]。目前,急性心肌梗死的病因学、治疗方案及正确地选择治疗药物已成为心血管疾病研究的重要课题。长期以来实验室研究是用大鼠制作急性心肌梗死模型,但是国内的急性心肌梗死模型的制作存在一定的缺陷和局限性。首先,大部分人是在人工呼吸机下制作急性心肌梗死模型,在气管切开时,易出血堵塞呼吸道,增加动物的死亡率。同时,由于呼吸机的型号、参数调整以及使用呼吸机时给动物带来的创伤等诸多因素,使得实验的重现性差,得出的数据可信程度降低;其次,使用呼吸机使实验的时间会大大延长,由于实验室的条件(包括仪器、环境和温度等)每天都会有所不同,从而增大了实验的系统误差和实验开支,使得实验很难开展。因此,为了避免上述缺点和局限性,我们采用非人工呼吸机下开胸结扎大鼠冠状动脉前降支造成急性心肌梗死模型,通过控制麻药的剂量、开胸的时间和减少出血等来提高大鼠的成活率,并且于急性心肌梗死6、12、24及48小时,通过心肌梗死面积、血清CK、LDH及AST活性、全血粘度和血浆粘度等指标确定非人工呼吸机下急性心肌梗死模型建立后各项指标的最佳检测时间。同时采用中药和西药进行药物干预,以进一步确证非人工呼吸机下急性心肌梗死模型建立的可靠性。
[Abstract]:The study of cardiovascular disease does not involve animal models. The use of animal models to mimic cardiovascular disease in humans not only avoids the risk of human trials, but also replicates models of disease at any time, which facilitates research and allows us to study the nature of the disease. To explore the pathogenesis of the disease and to find out appropriate treatment methods [1]. At present, the etiology of acute myocardial infarction (AMI), treatment regimen and correct choice of treatment drugs have become an important subject of cardiovascular disease research. For a long time, the rat model of acute myocardial infarction has been used in laboratory research, but there are some defects and limitations in the establishment of acute myocardial infarction model in China. First of all, most people make acute myocardial infarction model under artificial ventilator. During tracheotomy, it is easy to bleed and clog the respiratory tract and increase animal mortality. At the same time, because of many factors, such as the type of ventilator, the adjustment of parameters and the trauma caused by the use of ventilator, the reproducibility of the experiment is poor, and the reliability of the obtained data is reduced. Secondly, The use of ventilator can prolong the time of experiment greatly, because the conditions of laboratory (including instrument, environment and temperature etc.) will be different every day, thus increasing the systematic error of experiment and experiment expense, making the experiment very difficult to carry out. Therefore, in order to avoid the shortcomings and limitations mentioned above, we established the model of acute myocardial infarction by ligating the anterior descending branch of coronary artery under non-artificial ventilator in rats, by controlling the dosage of anesthetic. The time of thoracotomy and the reduction of bleeding were used to increase the survival rate of rats, and at 24 and 48 hours after acute myocardial infarction, the area of myocardial infarction, the activity of serum CKT LDH and AST were measured. The parameters of whole blood viscosity and plasma viscosity were used to determine the best detection time after the establishment of acute myocardial infarction model under non-artificial ventilator. Both traditional Chinese medicine and western medicine were used to further confirm the reliability of the model of acute myocardial infarction under non-artificial ventilator.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332;R542.22

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