中国台湾地区H6N1禽流感病毒血凝素基因的适应性进化

发布时间：2018-08-18 08:09

【摘要】：2013年在中国台湾地区发现了第一例人感染H6N1禽流感,研究该地区H6N1病毒血凝素基因(HA)的适应性进化特征和种群动力学,将为人感染H6N1病毒的来源、进化及致病机制的理解提供更多信息,为进一步的研究和防治提供一定帮助。本研究从Flu和GISAID两大数据库中获得中国台湾地区所有已释放的H6N1病毒的HA序列,构建系统发育树和进化动力曲线,并推测其进化速率,分析其适应性进化特征。结果表明中国台湾地区H6N1病毒的HA有五种类型,其中人感染H6N1病毒的HA所属类型为目前当地优势类型;该病毒种群于1971年底第一次扩张,2008年迅速减少,而后又略有增加。同时在长期的进化过程中,3个位点受到正选择作用的影响,增加了病毒的适应能力;89个位点受负选择作用的影响,它们在病毒的复制、流行中可能具有重要的功能;其中剪切位点附近的329位所受负选择作用强烈(PSLAC0.001、PFEL0.000、BFREL137),此位点可能与病毒毒力有关。中国台湾地区H6N1病毒的HA已发生了明显的适应性进化,具有发生疫情的可能性,人们应高度重视,并能及时给与监测和防治。
[Abstract]:The first case of human infection with H6N1 avian influenza was found in Taiwan in 2013. The study of the adaptive evolutionary characteristics and population dynamics of the (HA) gene of H6N1 virus hemagglutinin in this area will be the source of human H6N1 virus infection. The understanding of evolution and pathogenesis provides more information for further study and prevention. In this study, HA sequences of all released H6N1 viruses were obtained from Flu and GISAID databases in Taiwan, China. Phylogenetic trees and evolutionary dynamics curves were constructed, and their evolutionary rates were estimated and their adaptive evolution characteristics were analyzed. The results showed that there were five types of HA of H6N1 virus in Taiwan area of China. The HA of human infected with H6N1 virus belonged to the local dominant type, and the population of H6N1 virus expanded for the first time at the end of 1971, then decreased rapidly in 2008, and then increased slightly. At the same time, during the long evolutionary process, 3 sites were affected by positive selection, which increased the adaptability of virus, 89 sites were affected by negative selection, which may play an important role in virus replication and epidemic. The negative selection of 329 sites near the shear site (PSLAC0.001 PFEL0.000 BFREL137) may be related to the virulence of the virus. The HA of H6N1 virus in Taiwan area of China has undergone obvious adaptive evolution and has the possibility of outbreak. People should attach great importance to it and be able to monitor and prevent it in time.
【作者单位】：皖南医学院医学生物学教研室;
【基金】：省级优秀青年人才基金(WK200921) 校重点科研项目(WK2014Z02)
【分类号】：R373.9

【相似文献】