湖北土家族15个STR基因座遗传多态性研究
发布时间：2018-08-18 21:11
【摘要】： 目的:了解湖北土家族15个STR基因座(TH01、CSF1PO、HUMTPOX、FGA、vWA、D2S1338、D3S1358、D5S818、D7S820、D8S1179、D13S317、D16S539、D18S51、D19S433、D21S11)的遗传多态性,探讨该民族与国内18个民族群体的遗传关系。 材料与方法:湖北土家族无关个体血样110例,用Chelex-100方法提取DNA,应用AmpFl STR~(?)Identifiler~(Tm)kit荧光标记复合PCR扩增技术对样本DNA15个STR基因座进行扩增,在ABI Prism 3100型DNA序列分析仪对扩增产物进行检测,GeneScan Analysis3.7和Genotyper3.7分析软件对检测结果进行扫描分析。用人工方法(Excel软件)计算各个基因座的遗传多态性参数;另外选择与本研究9个STR基因座完全相同的18个国内民族群体进行群体遗传学研究,根据他们的等位基因频率算出遗传距离后,利用MEGA3.1软件的Neigllbour-Joining方法构建系统发生树。 结果:①遗传多态性参数:湖北土家族15个STR基因座中共检测出132种等位基因,等位基因频率分布在0.0045-0.5182之间;374种基因型,频率分布在0.0091～0.3182之间;杂合度(H_o)分布在0.6364～0.9091,平均杂合度为0.7873、多态信息含量(PIC)分布在0.5549～0.8552、个体识别力(DP)分布在0.7954～0.9595、累积个体识别力(TDP)大于0.99999999998,非父排除率(EP)分布在0.3658～0.8510,累积非父排除率(CEP)大于0.999999992。②群体遗传学研究:湖北土家族与地理位置位于其周边的汉族群体间的遗传距离普遍小于其与其他少数民族群体间的遗传距离,与维吾尔族的遗传距离最大。湖北土家族与其他18个民族群体的系统发生树结果显示湖北土家族首先和广西苗族、广西瑶族相聚,其次同四川汉族、江西汉族、浙江汉族、湖南汉族、河南汉族相聚,再次与安徽汉族、甘肃汉族、甘肃东乡族、陕西汉族、江苏汉族相聚,然后和拉萨藏族及云南彝族、云南纳西族相聚,最后与蒙古族、朝鲜族、维吾尔族相聚。 结论:湖北土家族的15个STR基因座除TPOX基因座外,其他14个基因座都属于高度多态性遗传标记。湖北土家族群体可能是以土家族的遗传标记为主的融合有其他民族遗传特征的群体。
[Abstract]:Objective: to investigate the genetic polymorphism of 15 STR loci in Tujia nationality of Hubei Province (TH01 / CSF1POP HUMTPOXA FGAvWAA D2S1338 / D3S1358 / D5S818D7S820 / D8S1179 / D16S539D18S51D19S433D21S11) and to explore the genetic relationship between this population and 18 ethnic groups in China. Materials and methods: one hundred and ten unrelated blood samples from Tujia nationality in Hubei Province were extracted by Chelex-100 method. The DNA15 STR loci were amplified by AmpFl STR ~ (?) Identifiler~ (Tm) kit fluorescence labeled PCR amplification technique. GeneScan Analysis3.7 and Genotyper3.7 software were used to detect the amplified products by ABI Prism 3100 DNA sequence analyzer. The genetic polymorphism parameters of each locus were calculated by artificial method (Excel software), and the population genetics of 18 national populations identical to 9 STR loci in this study were studied. The genetic distance was calculated according to their allelic frequency and the phylogenetic tree was constructed by Neigllbour-Joining method of MEGA3.1 software. Results 1 genetic polymorphism parameters: 132 alleles were detected in 15 STR loci of Tujia nationality in Hubei Province. The allele frequencies were between 0.0045-0.5182 and 0.3182, respectively. The distribution of heterozygosity (Ho) was 0.6364 / 0.9091, the average heterozygosity was 0.7873, the polymorphic information content (PIC) was 0.5549 / 0.8552, the individual recognition power (DP) was 0.7954 / 0.9595, the cumulative individual recognition power (TDP) was greater than 0.999999999998, the non-paternal exclusion rate (EP) was 0.36580.8510, and the cumulative non-paternal exclusion rate (EP) was 0.36580.8510. (CEP) > 0.9999992.2 population genetic study: the genetic distance between the Tujia nationality and the Han population located in its vicinity was generally smaller than the genetic distance between the Tujia nationality and the other minority populations in Hubei Province. The genetic distance between Uygur and Uygur is the largest. The phylogenetic tree results of the Tujia and other 18 ethnic groups in Hubei show that the Tujia in Hubei first meet with the Miao and Yao ethnic groups in Guangxi, then with the Han nationality in Sichuan, Jiangxi, Zhejiang, Hunan and Henan. They met with the Han nationality of Anhui, Gansu Dongxiang, Shaanxi Han and Jiangsu Han, then with Lhasa Tibetans, Yunnan Yi, Yunnan Naxi, and finally with Mongolian, Korean and Uygur. Conclusion: the 15 STR loci of Tujia nationality in Hubei are highly polymorphic except TPOX loci. The Tujia population in Hubei Province may be a population with genetic characteristics of other nationalities, which is based on genetic markers of Tujia nationality.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R394
本文编号：2190689
[Abstract]:Objective: to investigate the genetic polymorphism of 15 STR loci in Tujia nationality of Hubei Province (TH01 / CSF1POP HUMTPOXA FGAvWAA D2S1338 / D3S1358 / D5S818D7S820 / D8S1179 / D16S539D18S51D19S433D21S11) and to explore the genetic relationship between this population and 18 ethnic groups in China. Materials and methods: one hundred and ten unrelated blood samples from Tujia nationality in Hubei Province were extracted by Chelex-100 method. The DNA15 STR loci were amplified by AmpFl STR ~ (?) Identifiler~ (Tm) kit fluorescence labeled PCR amplification technique. GeneScan Analysis3.7 and Genotyper3.7 software were used to detect the amplified products by ABI Prism 3100 DNA sequence analyzer. The genetic polymorphism parameters of each locus were calculated by artificial method (Excel software), and the population genetics of 18 national populations identical to 9 STR loci in this study were studied. The genetic distance was calculated according to their allelic frequency and the phylogenetic tree was constructed by Neigllbour-Joining method of MEGA3.1 software. Results 1 genetic polymorphism parameters: 132 alleles were detected in 15 STR loci of Tujia nationality in Hubei Province. The allele frequencies were between 0.0045-0.5182 and 0.3182, respectively. The distribution of heterozygosity (Ho) was 0.6364 / 0.9091, the average heterozygosity was 0.7873, the polymorphic information content (PIC) was 0.5549 / 0.8552, the individual recognition power (DP) was 0.7954 / 0.9595, the cumulative individual recognition power (TDP) was greater than 0.999999999998, the non-paternal exclusion rate (EP) was 0.36580.8510, and the cumulative non-paternal exclusion rate (EP) was 0.36580.8510. (CEP) > 0.9999992.2 population genetic study: the genetic distance between the Tujia nationality and the Han population located in its vicinity was generally smaller than the genetic distance between the Tujia nationality and the other minority populations in Hubei Province. The genetic distance between Uygur and Uygur is the largest. The phylogenetic tree results of the Tujia and other 18 ethnic groups in Hubei show that the Tujia in Hubei first meet with the Miao and Yao ethnic groups in Guangxi, then with the Han nationality in Sichuan, Jiangxi, Zhejiang, Hunan and Henan. They met with the Han nationality of Anhui, Gansu Dongxiang, Shaanxi Han and Jiangsu Han, then with Lhasa Tibetans, Yunnan Yi, Yunnan Naxi, and finally with Mongolian, Korean and Uygur. Conclusion: the 15 STR loci of Tujia nationality in Hubei are highly polymorphic except TPOX loci. The Tujia population in Hubei Province may be a population with genetic characteristics of other nationalities, which is based on genetic markers of Tujia nationality.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R394
【引证文献】
相关硕士学位论文 前1条
1 范瑾;雪山蒙古族人群15个STR基因座遗传多态性[D];辽宁医学院;2012年
，本文编号：2190689
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