模拟高原低氧应激环境下大鼠内分泌代谢的变化研究
发布时间：2018-08-19 18:18
【摘要】：目的:探索高原低氧应激环境对大鼠糖脂代谢的影响。方法:将大鼠随机分为对照组和低压缺氧应激组,缺氧组大鼠置模拟海拔6 000m低压舱内48h×3次,实验结束后,缺氧组从低压氧舱中取出后立即断头处死,测定血糖,胰岛素,采用稳态模型HOMAβ评估胰岛β细胞功能,测定血脂(TC、TG、LDL-C、HDL-C),称量脏器重量,测定肝、肌糖原含量。结果:相比较正常组,Hypoxia组大鼠血糖值稍有降低,但胰岛素水平显著升高(P0.05)。Hypoxia组大鼠的HOMAβ高于正常对照组。脏器重量结果显示,Hypoxia组大鼠的肾脏重量显著降低(P0.05),脾脏、肝脏重量显著升高(P0.01,P0.05),肾上腺重量有下降趋势。与正常对照组相比,Hypoxia组大鼠TC含量显著增高(P0.01);Hypoxia组大鼠肝糖原显著升高(P0.05),而肌糖原显著降低(P0.01);Hypoxia组大鼠TRH、TSH、T3具有下降趋势,仅TSH具有统计学意义(P0.01),而T4显著升高(P0.05)。结论:高原低氧应激条件下,大鼠机体呈现血糖降低,胰岛功能增强,肝糖原储备增加,肌糖原储备减少,糖脂代谢紊乱。
[Abstract]:Objective: to explore the effect of high altitude hypoxia stress environment on glucose and lipid metabolism in rats. Methods: rats were randomly divided into control group and hypobaric stress group. Rats in hypoxia group were placed in a hypobaric chamber at a simulated altitude of 6 000 m for 48 h 脳 3 times. After the experiment, the rats in hypoxia group were taken out of hypobaric oxygen chamber and killed immediately, blood glucose and insulin were measured. The steady-state model HOMA 尾 was used to evaluate the function of islet 尾 cells, to measure serum lipids, to weigh organ weight, and to determine the content of glycogen in liver and muscle. Results: compared with the normal group, the blood glucose level in the Hypoxia group was slightly lower, but the insulin level was significantly increased (P0.05). The HOMA 尾 in the Hypoxia group was higher than that in the normal control group. The results of organ weight showed that the weight of kidney decreased significantly (P0.05), the weight of spleen and liver increased significantly (P0.01P05), and the weight of adrenal gland decreased in the group of Hypoxia. Compared with the normal control group, the TC content in the Hypoxia group was significantly higher (P0.01) and the hepatic glycogen level in the Hypoxia group was significantly higher (P0.05), while the muscle glycogen significantly decreased (P0.01) in the Hypoxia group, only TSH had statistical significance (P0.01), while T4 significantly increased (P0.05). Conclusion: under the condition of high altitude hypoxia stress, the blood glucose decreased, the islet function increased, the hepatic glycogen reserve increased, the muscle glycogen reserve decreased, and the metabolism of glucose and lipid was disturbed.
【作者单位】： 兰州军区兰州总医院内分泌科;兰州军区兰州总医院高原环境损伤防治重点实验室;
【基金】：国家自然科学基金资助项目(81173620,30772773)
【分类号】：R339.5
[Abstract]:Objective: to explore the effect of high altitude hypoxia stress environment on glucose and lipid metabolism in rats. Methods: rats were randomly divided into control group and hypobaric stress group. Rats in hypoxia group were placed in a hypobaric chamber at a simulated altitude of 6 000 m for 48 h 脳 3 times. After the experiment, the rats in hypoxia group were taken out of hypobaric oxygen chamber and killed immediately, blood glucose and insulin were measured. The steady-state model HOMA 尾 was used to evaluate the function of islet 尾 cells, to measure serum lipids, to weigh organ weight, and to determine the content of glycogen in liver and muscle. Results: compared with the normal group, the blood glucose level in the Hypoxia group was slightly lower, but the insulin level was significantly increased (P0.05). The HOMA 尾 in the Hypoxia group was higher than that in the normal control group. The results of organ weight showed that the weight of kidney decreased significantly (P0.05), the weight of spleen and liver increased significantly (P0.01P05), and the weight of adrenal gland decreased in the group of Hypoxia. Compared with the normal control group, the TC content in the Hypoxia group was significantly higher (P0.01) and the hepatic glycogen level in the Hypoxia group was significantly higher (P0.05), while the muscle glycogen significantly decreased (P0.01) in the Hypoxia group, only TSH had statistical significance (P0.01), while T4 significantly increased (P0.05). Conclusion: under the condition of high altitude hypoxia stress, the blood glucose decreased, the islet function increased, the hepatic glycogen reserve increased, the muscle glycogen reserve decreased, and the metabolism of glucose and lipid was disturbed.
【作者单位】： 兰州军区兰州总医院内分泌科;兰州军区兰州总医院高原环境损伤防治重点实验室;
【基金】：国家自然科学基金资助项目(81173620,30772773)
【分类号】：R339.5
【参考文献】
相关期刊论文 前3条
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