陈氏狸殖吸虫形态学及两种宿主适宜性的研究
发布时间：2018-08-20 14:21
【摘要】：[目的] 观察陈氏狸殖吸虫各期形态,并与斯氏狸殖吸虫的主要形态特征作比较;探讨陈氏狸殖吸虫与猫和大鼠的适宜性。 [方法]从陈氏狸殖吸虫流行区采集溪蟹,分离并观察囊蚴形态,人工感染实验动物猫1只和大鼠20只,猫经口感染,大鼠经口和腹腔注射感染.40d后检查粪便,观察排出虫卵的时间,90-112天内剖杀动物,回收虫体,计算获虫率及成虫率,并进行形态观察及比较。 [结果]陈氏狸殖吸虫的囊蚴、后尾蚴、成虫、虫卵的主要形态特征为:囊蚴的囊壁为单层、大小为0.445×0.440mm;后尾蚴的排泄囊为长袋形;成虫大小为10.12±0.88×3.34±0.65mm、宽长比例为1:2.44-1:3.2,腹吸盘大于口吸盘,卵巢有中心体、分4-6叶、呈指状分叶、其位置靠近腹吸盘,睾丸呈长形分叶、分4-6叶、长度约为体长的1/6,体棘为混生型;虫卵多数略不对称。实验猫粪检出现虫卵的时间为感染后第50d;大鼠阳性者4只,排卵时间分别为感染后第68、68、70、70d。猫的获虫率为20%,成虫率为87.5%;大鼠获虫率为6.67%,成虫率为43.75%。 [结论]陈氏狸殖吸虫的囊蚴及成虫形态具有特征性,其中囊蚴的囊壁层数及厚度、成虫卵巢的形态及体棘的排列是其与斯氏狸殖吸虫主要的区别点。陈氏狸殖吸虫与猫和大鼠的适宜性均比较好,这两种动物均可作为陈氏狸殖吸虫的实验终宿主。
[Abstract]:[objective] to observe the morphology of Pagumogonimus Chen in different stages and compare it with the main morphological characteristics of P. skrjabini, and to explore the suitability of the paragonimus Chen with cats and rats. [methods] Creek crabs were collected from the endemic areas of paragonimus chengii, and the morphology of cysticercaria was observed. One cat and 20 rats were artificially infected. The cats were infected by mouth, and faeces were examined by oral and intraperitoneal injection for 40 days. To observe the time of excretion of eggs within 90-112 days to kill animals, to recover the insect body, to calculate the rate of worm acquisition and adult, and to make morphological observation and comparison. [results] the main morphological characteristics of cysticercus, posterior cercariae, adults and eggs of Pagopogonimus Chen were as follows: the cyst wall of cysticercus was monolayer, the size was 0.445 脳 0.440 mm, the excretory cyst of posterior cercariae was long bag shape. The adult worm was 10.12 卤0.88 脳 3.34 卤0.65mm in size, with a width to length ratio of 1: 2.44-1: 3.2. the abdominal sucker was larger than the oral sucker, and the ovary had a central body. The ovary was divided into 4-6 leaves, which were finger lobed, and the testis were long lobed, 4-6 leaves, the length of the sucker was about 1 / 6 of body length, and the spines were mixed. Most eggs are slightly asymmetrical. The time of emergence of eggs in feces of experimental cats was the 50th day after infection, and the ovulation time of 4 positive rats was the 68th, 6870th and 70th day after infection, respectively. The rate of catching insects was 20 in cats and 87.5 in adults and 6.67 in rats and 43.75 in adults. [conclusion] the shape of cysticercaria and adults of Pagumogonimus Chen is characteristic. The number and thickness of cyst wall, the shape of ovary and the arrangement of body spine are the main distinguishing points between Cysticercus and paragonimus skrjabini. The suitability of paragonimus Chen is better than that of cat and rat. These two animals can be used as the final host of the experiment.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R383
本文编号：2193926
[Abstract]:[objective] to observe the morphology of Pagumogonimus Chen in different stages and compare it with the main morphological characteristics of P. skrjabini, and to explore the suitability of the paragonimus Chen with cats and rats. [methods] Creek crabs were collected from the endemic areas of paragonimus chengii, and the morphology of cysticercaria was observed. One cat and 20 rats were artificially infected. The cats were infected by mouth, and faeces were examined by oral and intraperitoneal injection for 40 days. To observe the time of excretion of eggs within 90-112 days to kill animals, to recover the insect body, to calculate the rate of worm acquisition and adult, and to make morphological observation and comparison. [results] the main morphological characteristics of cysticercus, posterior cercariae, adults and eggs of Pagopogonimus Chen were as follows: the cyst wall of cysticercus was monolayer, the size was 0.445 脳 0.440 mm, the excretory cyst of posterior cercariae was long bag shape. The adult worm was 10.12 卤0.88 脳 3.34 卤0.65mm in size, with a width to length ratio of 1: 2.44-1: 3.2. the abdominal sucker was larger than the oral sucker, and the ovary had a central body. The ovary was divided into 4-6 leaves, which were finger lobed, and the testis were long lobed, 4-6 leaves, the length of the sucker was about 1 / 6 of body length, and the spines were mixed. Most eggs are slightly asymmetrical. The time of emergence of eggs in feces of experimental cats was the 50th day after infection, and the ovulation time of 4 positive rats was the 68th, 6870th and 70th day after infection, respectively. The rate of catching insects was 20 in cats and 87.5 in adults and 6.67 in rats and 43.75 in adults. [conclusion] the shape of cysticercaria and adults of Pagumogonimus Chen is characteristic. The number and thickness of cyst wall, the shape of ovary and the arrangement of body spine are the main distinguishing points between Cysticercus and paragonimus skrjabini. The suitability of paragonimus Chen is better than that of cat and rat. These two animals can be used as the final host of the experiment.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R383
【引证文献】
相关期刊论文 前1条
1 戴婷婷;周本江;世淑兰;李翠英;张伟琴;王红;;云南省中西部两地并殖吸虫的种类与第二中间宿主调查[J];中国病原生物学杂志;2013年06期
相关硕士学位论文 前1条
1 戴婷婷;云南大理州并殖吸虫的病原学及分子系统学研究[D];昆明医科大学;2013年
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