高原创伤性颅脑损伤大鼠液压模型的实验研究
发布时间：2018-08-21 07:49
【摘要】： 目的:为建立动物颅脑损伤实验平台,提高研究质量,本研究拟根据液压传递实验原理,设计制作液压打击装置,并明确操作规范;以此建立可以量化分级的液压损伤大鼠创伤性颅脑损伤(TBI)模型;对高原环境下液压损伤大鼠TBI动物模型进行初步的研究。 方法:1、依据液压传递原理及加速性颅脑损伤原理,设计制作能根据需要调节输出不同打击力度的动物液压损伤打击装置;2、建立可以分级的液压损伤大鼠TBI模型:32只成年SD大鼠分为2组:对照组(n=8)和损伤组(n=24)。损伤组又分3个亚组,每亚组各8只大鼠。分别给予0.5-1.0atm、1.5-2.0atm、3.0-3.5atm的压强打击致伤后作动物行为和病理学评估。3、不同海拔环境下大鼠颅脑液压损伤后比较观察:60只成年SD大鼠分为2组:低海拔组(n=30)和高海拔组(n=30)。每组分为对照组(n=6)和损伤组(n=24);每个损伤组按时间点又分4个亚组,每亚组各6只大鼠。损伤组和对照组都进行规范化的手术步骤。对照组不进行液压打击致伤,损伤组给予约1.5atm打击压强造成中度液压损伤大鼠TBI模型,按时间点分别处理大鼠并取样,比较不同海拔环境条件下颅脑损伤动物行为学及病理学改变,并观察其微循环的变化特征。 结果:本研究成功制作了动物液压打击装置,在摆锤释放的角度逐渐增加时,产生的压强也逐渐增加,而同一角度释放摆锤产生的压强基本一致。利用该液压打击装置建立了液压损伤大鼠TBI动物模型,在不同的打击压强下造成的病理改变不同,随着打击压强的增加,造成大鼠的神经运动功能障碍,组织病理改变,脑组织含水量的异常也加重。与平原环境组相比,高原环境下液压打击造成的TBI大鼠病理改变、神经功能障碍明显;脑含水量和vWF改变与平原环境组相比差异具有统计学显著性。 结论: 1、本研究制作的液压打击装置性能稳定,操作简便,可以较好的应用于动物的颅脑损伤实验研究。2、利用该液压打击装置建立的液压损伤大鼠TBI模型,可以较好的模拟临床TBI改变。3、本实验首次建立了高原环境下液压损伤大鼠TBI模型,为进一步开展高原颅脑损伤研究提供了一个可靠的实验平台。
[Abstract]:Objective: in order to establish the experimental platform of animal craniocerebral injury and improve the research quality, according to the principle of hydraulic transmission experiment, this study designed and made hydraulic hitting device, and defined the operation standard; The (TBI) model of traumatic craniocerebral injury in rats with hydraulic injury was established, and the TBI animal model of hydraulic injury in high altitude environment was preliminarily studied. Methods: according to hydraulic transfer principle and accelerated craniocerebral injury principle, An animal hydraulic injury attack device was designed and manufactured according to the need to adjust the output of different attack forces. The TBI model of rats with hydraulic injury was established. 32 adult SD rats were divided into two groups: control group (nong8) and injury group (nong24), which could be divided into two groups: control group (n = 8) and injury group (n = 24). The injury group was divided into 3 subgroups with 8 rats in each subgroup. The animal behavior and pathology were evaluated by 0.5-1.0atmAm 1.5-2.0atm-1. 0-3.5atm. After craniocerebral hydraulic injury, 60 adult Sprague-Dawley rats were divided into two groups: low altitude group (nun30) and high altitude group (nun30), and the rats were divided into two groups: low altitude group (n = 30) and high altitude group (n = 30). Each group was divided into two groups: control group (n = 6) and injury group (n = 24), and each group was divided into 4 subgroups (n = 6) and each subgroup (n = 6). Both the injury group and the control group underwent standardized surgical procedures. The TBI model of moderate hydraulic injury was induced by 1.5atm shock pressure in the injury group. The rats were treated and sampled according to the time points. To compare the behavioral and pathological changes of craniocerebral injury at different altitude, and to observe the characteristics of microcirculation. Results: the animal hydraulic impact device was successfully manufactured. When the angle of the pendulum hammer release increased gradually, the pressure produced by the pendulum hammer increased gradually, and the pressure produced by the same angle release pendulum hammer was basically the same. The TBI animal model of rats with hydraulic injury was established by using the device. The pathological changes were different under different impact pressures. With the increase of the impact pressure, the neurological motor dysfunction and histopathological changes were induced in rats. The abnormality of water content in brain tissue is also aggravated. Compared with plain environment group, the pathological changes of TBI rats caused by hydraulic attack under high altitude environment were obvious, and the changes of brain water content and vWF were significantly different from those of plain environment group. Conclusion: 1, the hydraulic hitting device made in this study is stable in performance, easy to operate, and can be used in the experimental study of animal craniocerebral injury. The TBI model of rat with hydraulic injury was established by using the hydraulic hitting device. The model of TBI in rats with hydraulic injury under high altitude environment was established for the first time, which provided a reliable experimental platform for further research on craniocerebral injury at high altitude.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R651.15;R-332
[Abstract]:Objective: in order to establish the experimental platform of animal craniocerebral injury and improve the research quality, according to the principle of hydraulic transmission experiment, this study designed and made hydraulic hitting device, and defined the operation standard; The (TBI) model of traumatic craniocerebral injury in rats with hydraulic injury was established, and the TBI animal model of hydraulic injury in high altitude environment was preliminarily studied. Methods: according to hydraulic transfer principle and accelerated craniocerebral injury principle, An animal hydraulic injury attack device was designed and manufactured according to the need to adjust the output of different attack forces. The TBI model of rats with hydraulic injury was established. 32 adult SD rats were divided into two groups: control group (nong8) and injury group (nong24), which could be divided into two groups: control group (n = 8) and injury group (n = 24). The injury group was divided into 3 subgroups with 8 rats in each subgroup. The animal behavior and pathology were evaluated by 0.5-1.0atmAm 1.5-2.0atm-1. 0-3.5atm. After craniocerebral hydraulic injury, 60 adult Sprague-Dawley rats were divided into two groups: low altitude group (nun30) and high altitude group (nun30), and the rats were divided into two groups: low altitude group (n = 30) and high altitude group (n = 30). Each group was divided into two groups: control group (n = 6) and injury group (n = 24), and each group was divided into 4 subgroups (n = 6) and each subgroup (n = 6). Both the injury group and the control group underwent standardized surgical procedures. The TBI model of moderate hydraulic injury was induced by 1.5atm shock pressure in the injury group. The rats were treated and sampled according to the time points. To compare the behavioral and pathological changes of craniocerebral injury at different altitude, and to observe the characteristics of microcirculation. Results: the animal hydraulic impact device was successfully manufactured. When the angle of the pendulum hammer release increased gradually, the pressure produced by the pendulum hammer increased gradually, and the pressure produced by the same angle release pendulum hammer was basically the same. The TBI animal model of rats with hydraulic injury was established by using the device. The pathological changes were different under different impact pressures. With the increase of the impact pressure, the neurological motor dysfunction and histopathological changes were induced in rats. The abnormality of water content in brain tissue is also aggravated. Compared with plain environment group, the pathological changes of TBI rats caused by hydraulic attack under high altitude environment were obvious, and the changes of brain water content and vWF were significantly different from those of plain environment group. Conclusion: 1, the hydraulic hitting device made in this study is stable in performance, easy to operate, and can be used in the experimental study of animal craniocerebral injury. The TBI model of rat with hydraulic injury was established by using the hydraulic hitting device. The model of TBI in rats with hydraulic injury under high altitude environment was established for the first time, which provided a reliable experimental platform for further research on craniocerebral injury at high altitude.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R651.15;R-332
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