双歧杆菌与乳酸杆菌混合发酵条件的优化研究

发布时间：2018-08-21 13:41

【摘要】：本文通过正交试验,首先优化双歧杆菌和乳酸杆菌混合发酵工艺,确定得出其最适发酵条件为:初始pH值为7.2,培养温度为35℃,最佳培养基配方为酵母膏0.9%,胰蛋白胨0.6%,大豆蛋白胨0.3%,葡萄糖2.5%,双歧因子(低聚果糖)0.3%,生长因子5%。在此基础上,在100L发酵罐规模,进行了乳酸菌和双歧杆菌通过上述条件混合发酵中实验试,实验结果显示,发酵液中双歧杆菌、乳酸杆菌发酵液的活菌数均达到每mL在10亿/mL个以上,可以满足产业化需要。
[Abstract]:In this paper, the optimum fermentation conditions of bifidobacterium and lactobacillus were determined by orthogonal experiment. The optimum fermentation conditions were as follows: initial pH value was 7.2, culture temperature was 35 鈩，

本文编号：2195911