姜黄素诱导肿瘤细胞自噬后的自噬体浓缩疫苗抗肿瘤的机制研究
发布时间：2018-08-22 18:27
【摘要】：目的研究姜黄素诱导肿瘤细胞自噬后的自噬体浓缩疫苗抗肿瘤的机制。方法细胞自噬通过Western blotting和透射电子显微镜。小鼠骨髓来源的树突状细胞(BMDCs)的成熟表型经常规扫描电子显微镜(SEM)、流式细胞仪(FCM)和透射电子显微镜(TEM)证实。自噬体介导的交叉呈递效率通过羟基荧光素二醋酸盐琥珀酰亚胺脂(CFSE)的分布测定T细胞的增殖。分析自噬体的抗原交叉呈递的机理。结果姜黄素诱导的自噬通过自噬体的电子显微镜以及LC3-Ⅱ的表达所证实。自噬体浓缩疫苗上调表达CD40、CD86和MHCⅡ。经自噬体介导的T细胞激活后,T细胞有明显的扩增同时能杀伤靶细胞。HSP90、HSP70和HMGB1在这种新的疫苗中发挥重要作用。结论这些数据表明以自噬体为基础的疫苗有抗肿瘤疗效独特的特点。这种新的肿瘤疫苗的功效需临床试验的进一步验证。
[Abstract]:Objective to study the mechanism of anti-tumor induced by curcumin-induced autophagy concentrated vaccine. Methods autophagy was carried out by Western blotting and transmission electron microscope (TEM). The mature phenotype of murine bone marrow-derived dendritic cells (BMDCs) was confirmed by conventional scanning electron microscopy (SEM) (SEM), flow cytometry (FCM) and transmission electron microscopy (TEM). The cross-presentation efficiency mediated by autophagy was determined by the distribution of hydroxyfluorescein diacetate succinimide (CFSE). The mechanism of antigen cross-presentation of autophagy was analyzed. Results curcumin induced autophagy was confirmed by electron microscopy and LC3-鈪，
本文编号：2197936
[Abstract]:Objective to study the mechanism of anti-tumor induced by curcumin-induced autophagy concentrated vaccine. Methods autophagy was carried out by Western blotting and transmission electron microscope (TEM). The mature phenotype of murine bone marrow-derived dendritic cells (BMDCs) was confirmed by conventional scanning electron microscopy (SEM) (SEM), flow cytometry (FCM) and transmission electron microscopy (TEM). The cross-presentation efficiency mediated by autophagy was determined by the distribution of hydroxyfluorescein diacetate succinimide (CFSE). The mechanism of antigen cross-presentation of autophagy was analyzed. Results curcumin induced autophagy was confirmed by electron microscopy and LC3-鈪，
本文编号：2197936
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