引发医院感染表皮葡萄球菌对大环内酯—林可酰胺—链阳菌素B（MLS_B）耐药机制的研究
发布时间：2018-08-23 14:58
【摘要】：表皮葡萄球菌(以下简称表葡菌)是引发医院感染的重要致病菌，已得到人们的广泛关注。为进一步深入了解引发医院感染的表葡菌对大环内酯-林可酰胺-链阳菌素B(MLS_B)的耐药机制，本研究收集了2003～2004年北京3家综合性医院126株引发医院感染的表葡菌，检测了红霉素(ERY)、克林霉素(CLI)、克拉霉素(CLR)、头孢西丁(FOX)和喹奴普丁／达福普丁对这些菌株的最小抑菌浓度(MIC)；用D试验区分诱导型(iMLS_B)和泵出型(MS)耐药菌株：采用PCR法对耐药基因ermA、ermB、ermC、msrA和mecA进行了检测，，并用脉冲场凝胶电泳(PFGE)进行同源性分析。结果发现绝大多数的菌株对大环内酯类和林可酰胺类抗生素耐药，但对喹奴普丁／达福普丁敏感：结构型(cMLS_B)、诱导型(iMLS_B)和泵出型(MS)MLS_B耐药菌株分别占73.8％、10.3％和8.7％：在cMLS_B中，耐甲氧西林表葡菌(MRSE)占的比例较高，而在iMLS_B中甲氧西林敏感株(MSSE)较多；携带ermA、ermB和ermC的菌株分别占4.27％、2.6％和80.3％，ermC不仅在MRSE和MSSE中是最常见的耐药基因，而
[Abstract]:Staphylococcus epidermidis (Staphylococcus epidermidis) is an important pathogen causing nosocomial infection. In order to further understand the drug resistance mechanism of nosocomial infection induced by Staphylococcus epibacteria to macrolide-linamide-streptavidin B (MLS_B), 126 strains of S. epidermidis causing nosocomial infection were collected from 2003 to 2004 in 3 general hospitals in Beijing. The minimal inhibitory concentrations (MIC);) of erythromycin (ERY), clindamycin, (CLI), clarithromycin, (CLR), cefoxitin (FOX) and quinuptin / dafopudine against these strains were determined. IMLS_B and (MS) resistant strains were detected by PCR method, and the homology analysis was carried out by pulsed field gel electrophoresis (PFGE). The results showed that most of the strains were resistant to macrolides and lincomas, but sensitive to quinopudine / dafopudine: structural (cMLS_B), inducible (iMLS_B) and pump type (MS) MLS_B) resistant strains accounted for 73. 8% and 8. 7%, respectively. The proportion of methicillin-resistant Staphylococcus epidermidis (MRSE) was higher, but the number of methicillin-sensitive strains (MSSE) was higher in iMLS_B, and that of ermAmin-ermB and ermC were 4.27.6% and 80.3%, respectively, which were not only the most common resistant genes in MRSE and MSSE, but also in MRSE and MSSE.
【学位授予单位】：第四军医大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R378
本文编号：2199415
[Abstract]:Staphylococcus epidermidis (Staphylococcus epidermidis) is an important pathogen causing nosocomial infection. In order to further understand the drug resistance mechanism of nosocomial infection induced by Staphylococcus epibacteria to macrolide-linamide-streptavidin B (MLS_B), 126 strains of S. epidermidis causing nosocomial infection were collected from 2003 to 2004 in 3 general hospitals in Beijing. The minimal inhibitory concentrations (MIC);) of erythromycin (ERY), clindamycin, (CLI), clarithromycin, (CLR), cefoxitin (FOX) and quinuptin / dafopudine against these strains were determined. IMLS_B and (MS) resistant strains were detected by PCR method, and the homology analysis was carried out by pulsed field gel electrophoresis (PFGE). The results showed that most of the strains were resistant to macrolides and lincomas, but sensitive to quinopudine / dafopudine: structural (cMLS_B), inducible (iMLS_B) and pump type (MS) MLS_B) resistant strains accounted for 73. 8% and 8. 7%, respectively. The proportion of methicillin-resistant Staphylococcus epidermidis (MRSE) was higher, but the number of methicillin-sensitive strains (MSSE) was higher in iMLS_B, and that of ermAmin-ermB and ermC were 4.27.6% and 80.3%, respectively, which were not only the most common resistant genes in MRSE and MSSE, but also in MRSE and MSSE.
【学位授予单位】：第四军医大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R378
【参考文献】
相关期刊论文 前1条
1 熊自忠,李涛,徐元宏;临床分离耐甲氧西林表皮葡萄球菌的耐药性监测[J];中国医师杂志;2005年02期
本文编号：2199415
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