IL-18协同单纯疱疹病毒Ⅰ型糖蛋白B基因疫苗的免疫学研究

发布时间：2018-08-27 09:35

【摘要】： 目的:探讨IL-18分子佐剂在增强gB核酸疫苗抵抗HSV-1感染的免疫保护的作用,为研制新型HSV-1核酸疫苗奠定理论基础。方法:昆明小鼠随机分为三组,分别接种pcDNA3、pgB和pgB+pIL-18 DNA疫苗,每次接种质粒100μg,每次间隔2周,第3次免疫后2周,用ELISA检测特异性抗体滴度;利用~3H-TdR掺入法进行T细胞特异性抗原刺激实验;耳廓肿胀实验检测迟发型超敏反应(DTH反应);角膜攻击实验检测核酸疫苗肌肉接种小鼠后对小鼠的保护效果。结果:与pgB单独免疫组相比,pIL-18的协同免疫可以显著增强ELISA特异性抗体滴度、血清特异性中和抗体滴度、T细胞增殖反应和DTH反应,在动物保护实验中,pIL-18的协同免疫明显增强了pgB疫苗在病毒攻击时对角膜的保护效果。结论:pgB接种小鼠后可以诱导产生特异性体液免疫和细胞免疫,并具有免疫保护作用;pIL-18的协同免疫可以显著提高pgB DNA疫苗诱导的体液免疫和细胞免疫水平,增强核酸疫苗对机体的免疫保护作用。为进一步构建重组疫苗并最终用于单疱病毒性角膜炎的预防奠定理论基础。
[Abstract]:Objective: To investigate the role of IL-18 molecular adjuvant in enhancing the immune protection of gB nucleic acid vaccine against HSV-1 infection, and to lay a theoretical foundation for the development of a new type of HSV-1 nucleic acid vaccine.
Methods: Kunming mice were randomly divided into three groups, inoculated with pcDNA3, pgB and pgB + pIL-18 DNA vaccine, each time 100 UG plasmid was inoculated, 2 weeks interval, 2 weeks after the third immunization, specific antibody titer was detected by ELISA, T cell specific antigen stimulation test was performed by ~3H-TdR incorporation method, delayed type hypersensitivity (DTH reverse) was detected by auricle swelling test. The corneal attack test was used to detect the protective effect of nucleic acid vaccine on mice after inoculation.
Results: Compared with pgB alone immunization group, the synergistic immunization of pIL-18 could significantly enhance the titer of ELISA-specific antibody, serum-specific neutralizing antibody, T cell proliferation and DTH reaction. In animal protection experiments, the synergistic immunization of pIL-18 significantly enhanced the protective effect of pgB vaccine on cornea during viral attack.
CONCLUSION: The specific humoral and cellular immunity can be induced by pgB vaccination in mice, and the synergistic immunization of pIL-18 can significantly improve the humoral and cellular immunity induced by pgB DNA vaccine and enhance the immune protection of nucleic acid vaccine. It lays a theoretical foundation for the prevention of herpes simplex keratitis.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392

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