巴马壮族长寿人群转化生长因子-β1基因多态性的研究
发布时间：2018-08-28 19:00
【摘要】： 目的:探讨转化生长因子-β1(TGF-β1)基因的多态性与人类长寿的相关性。方法:采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术,检测132例广西巴马地区健康壮族长寿老人TGF-β1的基因多态性,包括TGF-β1基因启动子区域G-800A、C-509T和第一外显子T+869C、G+915C四个多态位点,并与118例巴马地区健康壮族成年人和70例广西贺州地区健康壮族人群作比较。结果:1.TGF-β1基因的G-800A、G+915C两个多态位点在广西巴马地区及贺州地区未检测出多态性;2.TGF-β1基因的C-509T、T+869C两个多态位点的等位基因频率和基因型频率分布比较分析显示,巴马长寿组与对照组之间无显著性差异(P＞0.05),而巴马长寿组与贺州壮族组(P_(-509)~(allele)=0.000,P_(-509)~(genotype)=0.000,P_(+869)~(allele)=0.000,P_(+869)~(genotype)=0.003)、巴马对照组与贺州壮族组(P_(-509)~(allele)=0.034,P_(-509)~(genotype)=0.039,P_(+869)~(allele)=0.017,P_(+869)~(genotype)=0.026)之间均有显著性差异;3.进一步的基因型组合分析显示,TGF-β1基因G-800A、C-509T、T+869C、G+915C四个多态位点基因型组合GG-CT-TT-GG在巴马长寿老人组中的分布明显高于巴马对照组(P=0.000);基因型组合GG-CT-TC-GG和GG-TT-CC-GG在巴马长寿组和贺州壮族组(P_(GG-CT-TC-GG)=0.002,P_(GG-TT-CC-GG)=0.002)、巴马对照组和贺州壮族组(P_(GG-CT-TC-GG)=0.002,P_(GG-TT-CC-GG)=0.031)之间均存在显著差异。结论:巴马地区壮族长寿人群TGF-β1基因某些多态位点的等位基因频率、基因型频率及基因型组合频率有异于普通人群,与该地区的长寿呈一定的相关性,提示巴马地区长寿人群存在独特的抗炎性衰老机制,从而在一定程度上体现出生存优势。
[Abstract]:Objective: to investigate the association between the polymorphism of transforming growth factor- 尾 1 (TGF- 尾 1) gene and longevity. Methods: polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the gene polymorphism of TGF- 尾 1 in 132 healthy Zhuang people in Guangxi. Four polymorphic loci including G-800AnC-509T and G915C in the promoter region of TGF- 尾 1 gene were compared with 118 healthy adults in Bama area and 70 healthy Zhuang people in Hezhou area of Guangxi. Results 1. Two polymorphic loci of TGF- 尾 1 gene G-800Agng G915C were not detected in Bama and Hezhou regions of Guangxi. 2. The allele frequency and genotype frequency distribution of the C-509TF- T869C locus of TGF- 尾 1 gene were compared and analyzed. 宸撮┈闀垮缁勪笌瀵圭収缁勪箣闂存棤鏄捐憲鎬у樊寮，
本文编号：2210312
[Abstract]:Objective: to investigate the association between the polymorphism of transforming growth factor- 尾 1 (TGF- 尾 1) gene and longevity. Methods: polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the gene polymorphism of TGF- 尾 1 in 132 healthy Zhuang people in Guangxi. Four polymorphic loci including G-800AnC-509T and G915C in the promoter region of TGF- 尾 1 gene were compared with 118 healthy adults in Bama area and 70 healthy Zhuang people in Hezhou area of Guangxi. Results 1. Two polymorphic loci of TGF- 尾 1 gene G-800Agng G915C were not detected in Bama and Hezhou regions of Guangxi. 2. The allele frequency and genotype frequency distribution of the C-509TF- T869C locus of TGF- 尾 1 gene were compared and analyzed. 宸撮┈闀垮缁勪笌瀵圭収缁勪箣闂存棤鏄捐憲鎬у樊寮，
本文编号：2210312
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