威海地区幽门螺杆菌cagA基因3′端多态性分析

发布时间：2018-08-29 11:48

【摘要】：目的研究威海地区幽门螺杆菌(Helicobacter pylori,Hp)临床分离株cagA基因的3′端多态性。方法采用PCR法扩增Hp临床分离株的cagA基因3′端并测序,用DNAStar 5.0软件对其进行编码氨基酸序列的比对分析。结果 63株Hp临床分离株均cagA阳性(cagA+),其中93.7%(59/63)为东亚型(EPIYYA-ABD),6.3%(4/63)为西方型(EPIYYA-ABC)。3.4%(2/59)的东亚型菌株EPIYA-B突变为ESIYA-B,4株西方型型均突变为EPIYT-B。胃癌患者分离株中88.9%(16/18)为东亚型,11.1%(2/18)为西方型;非胃癌分离株中95.6%(43/45)为东亚型,4.4%(2/45)为西方型。2株分离自胃癌患者的西方型Hp EPIYA-A后的第一个氨基酸残基由赖氨酸(K)突变为谷氨酸(E)。结论威海地区Hp cagA基因以东亚型为主,但东、西方亚型Hp的致病性无显著差别,两型都存在cagA基因3′端编码氨基酸序列的改变。
[Abstract]:Objective to study the 3 'terminal polymorphism of cagA gene in clinical isolates of Helicobacter pylori (Helicobacter pylori,Hp) in Weihai area. Methods the 3 'end of cagA gene of Hp clinical isolates was amplified by PCR and sequenced. The coding amino acid sequence was analyzed by DNAStar 5.0 software. Results of 63 clinical isolates of Hp, 93% (59 / 63) of them were cagA positive (cagA), of which 93 7% (59 / 63) were EPIYYA-ABD and 6.3% (4 / 63) were EPIYYA-ABC. 3. 4% (2 / 59) East Asian EPIYA-B mutated to ESIYA-B,4. Western type were all mutated to EPIYT-B.. 88. 9% (16 / 18) of gastric cancer isolates were East Asian type (2 / 18) and 95. 6% (43 / 45) of non-gastric cancer isolates were East Asian type 4. 4% (2 / 45). The first amino acid residues were mutated from lysine (K) to glutamate (E). 2. 2% (2 / 45) of western Hp EPIYA-A isolated from gastric cancer patients. Conclusion the Hp cagA gene in Weihai area is mainly East Asia type, but the pathogenicity of Hp subtype in east and west has no significant difference. The 3 '-terminal amino acid sequence of cagA gene is changed in both types.
【作者单位】：滨州医学院病原生物学教研室;滨州医学院附属医院呼吸内科;
【基金】：国家自然科学基金项目(No.81072429) 教育部“新世纪优秀人才支持计划”项目(No.NCET-11-1026)
【分类号】：R378.911

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