复方鹿角霜对胶原诱导性关节炎模型小鼠抗炎作用研究

发布时间：2018-08-30 10:52

【摘要】：目的观察复方鹿角霜对小鼠胶原诱导性关节炎(CIA)体内相关炎症细胞因子的表达及滑膜组织病理学的影响。方法建立小鼠CIA模型,实验小鼠随机分为5组:空白对照组,模型对照组,复方鹿角霜总膏低、高剂量组(C1,C2组),雷公藤多苷片组。小鼠第一次免疫后,观察小鼠的一般情况,记录小鼠前后足爪病变发生情况。小鼠第二次免疫后,复方鹿角霜C1、C2组分别灌胃给予复方鹿角霜总膏2.5,5 g·kg-1·d-1,雷公藤多苷片组灌胃给予雷公藤多苷片13.6 g·kg-1·d-1,连续4周。小鼠给药结束后,取血分离血清,酶联免疫吸附试验(ELISA)检测小鼠血清肿瘤坏死因子-α(TNF-α)和白细胞介素-4(IL-4)的表达水平。取小鼠足踝关节,光镜观察关节滑膜组织病理学变化。结果与模型对照组比较,复方鹿角霜C1、C2组、雷公藤多苷片组血清TNF-α均明显降低;而复方鹿角霜组血清IL-4水平升高。模型对照组关节病理可见滑膜组织增生,各给药组关节病理形态有所改善。结论复方鹿角霜可减轻胶原诱发的小鼠关节炎红肿症状,其作用机制可能与降低炎性因子TNF-α、调节抑炎因子IL-4作用有关。
[Abstract]:Objective to observe the effect of compound Lujiao cream on the expression of inflammatory cytokines and histopathology of synovium in (CIA) of mice with collagen-induced arthritis. Methods CIA model was established in mice. The experimental mice were randomly divided into 5 groups: blank control group, model control group, compound Lujiao cream low, high dose group (C1C 2 group), Tripterygium wilfordii polyglycoside group. After the first immunization, the general situation of mice was observed and the occurrence of paw lesions before and after immunization was recorded. After the second immunization, the compound antler cream C _ 1C _ 2 group was given the compound antler cream 2.5g kg-1 d ~ (-1) by gavage, and the tripterygium wilfordii polyglycoside tablet 13.6 g kg-1 d ~ (-1) by gavage for 4 weeks. Serum samples were collected from mice after administration, and the expression of tumor necrosis factor- 伪 (TNF- 伪) and interleukin-4 (IL-4) in serum of mice were detected by Elisa (ELISA). The serum levels of tumor necrosis factor 伪 (TNF- 伪) and interleukin-4 (IL-4) in mice were determined by enzyme-linked immunosorbent assay. The histopathological changes of synovial membrane were observed under light microscope. Results compared with the model control group, the serum TNF- 伪 of the C _ 1C _ 2 group and the Tripterygium wilfordii polyglycoside group were significantly decreased, while the serum IL-4 level of the compound antler cream group was higher than that of the control group. Synovial tissue proliferation was observed in the model control group, and the joint morphology was improved in each administration group. Conclusion compound Lujiao cream can alleviate the symptoms of arthritis redness and swelling induced by collagen in mice, and its mechanism may be related to the reduction of inflammatory factor TNF- 伪 and the regulation of anti-inflammatory factor IL-4.
【作者单位】：三峡大学第一临床医学院;湖北省宜昌市中心人民医院药剂科;华中科技大学同济医学院药学院;湖北中医药大学;
【基金】：国家“重大新药创制”科技重大专项项目(2011ZX09102-006-05)
【分类号】：R285.5;R-332

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