大鼠前列腺伤害性刺激时脊髓、孤束核P2X3受体、Fos的表达

发布时间：2018-08-30 16:38

【摘要】：目的:研究大鼠前列腺伤害性刺激与P2X3受体、Fos在脊髓内、孤束核的表达及其意义。方法:建立大鼠前列腺伤害性刺激动物模型,按完全随机化设计,三因素三水平析因试验,因素水平共有27种组合,健康雄性Wistar大鼠54只随机分为27组,每组2只,分别给予上述27种处理。如27号鼠接受三种因素的第三水平处理,即:腹正中切口,剪断腹下丛;腹正中切口,直视下向前列腺两叶各注射大肠杆菌(0∶1H∶2Z17)悬液0.1ml(浓度1×10~8个/ml);干预后存活72小时。余者类推。用抗Fos蛋白和抗P2X3受体的免疫组织化学方法,对大鼠前列腺伤害性刺激后脊髓内、孤束核内Fos、P2X3受体免疫阳性物表达部位进行观察。结果:在脊髓L_1水平切面上,可见Fos免疫阳性细胞主要分布在脊髓背角Ⅰ、Ⅱ板层,后联合、中央管周围和前角也有分布。P2X3受体阳性细胞主要见于脊髓背角Ⅱ板层。孤束核有不同程度的Fos、P2X3受体免疫阳性细胞表达(P0.05)。前列腺的交感通路切断后,L_1水平的Fos、P2X3受体免疫阳性细胞数明显下降(P0.05)。2%甲醛液引起的P2X3受体免疫阳性细胞数比大肠杆菌引起的多(P0.05)。结论:交感神经通路在大鼠前列腺伤害性信息传递过程中具有重要作用;P2X3受体可能参与了前列腺伤害性刺激的传导调制;孤束核可能参与了前列腺伤害性刺激信息的调制。
[Abstract]:Aim: to study the expression and significance of nociceptive stimulation and P2X3 receptor Fos in spinal cord of rats. Methods: an animal model of noxious prostatic stimulation was established in rats. According to the totally randomized design, three factors and three levels of factorial test, there were 27 combinations at the factor level. 54 healthy male Wistar rats were randomly divided into 27 groups with 2 rats in each group. 27 treatments were given respectively. For example, 27 rats were treated at the third level of three kinds of factors, that is, median abdominal incision, cut off the lower abdominal plexus, median abdominal incision, injection of Escherichia coli (0:1H:2Z17) suspension 0.1ml (1 脳 108 / ml) into each of the two lobes of prostate directly, and survived 72 hours after intervention. The rest are analogies. The immunoreactive sites of Fos,P2X3 receptors in the spinal cord and nucleus tractus solitarius after noxious stimulation of rat prostate were observed by immunohistochemical method of anti Fos protein and anti P2X3 receptor. Results: on the L1 level of spinal cord, it was found that Fos immunoreactive cells were mainly distributed in the lamina 鈪，

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