结核分枝杆菌16ku、38ku蛋白基因的克隆表达及其初步应用

发布时间：2018-09-01 08:20

【摘要】：1882年Koch发现结核分枝杆菌(MTB)以来,至今已有一个多世纪,但该菌仍是严重危害人类健康的公共卫生问题。结核病被世界卫生组织列为重点控制疾病之一。结核病由结核杆菌引起,结核杆菌是分枝杆菌属内对人类(及动物)致病的主要病原菌,其感染发病率最高,占90%左右。近年来全球感染结核分支杆菌的趋势严重上升,每年新发病例约有800—1000万,为传染病中的“头号杀手”。对结核分支杆菌的预防,诊断和治疗的研究已成为当务之急。长期以来诊断结核病是用痰标本的抗酸染色或MTB的细菌培养,结合患者临床症状及胸部X片。但是这些方法由于MTB自身的生长特性缺乏敏感性,尤其是用于诊断肺外结核。目前对结核病临床可用的筛选方法虽采用ELISA法,斑点吸附免疫金抗法,但所用包被抗原多为粗制抗原,如:聚合OT、PPD、BCG、半糖脂抗原,使所测得的结果不稳定,使临床对肺外结核与菌培养阴性结核的诊断带来困难。据Ivanyi研究发现以结核分支杆菌单克隆抗体筛选出来的抗原仅
[Abstract]:It has been more than a century since (MTB) was discovered by Koch in 1882, but it is still a serious public health problem. Tuberculosis is listed by the World Health Organization as one of the key diseases under control. Tuberculosis is caused by Mycobacterium tuberculosis. Mycobacterium tuberculosis is the main pathogen in the genus Mycobacterium that causes disease to human (or animal). The incidence of tuberculosis is the highest (about 90%). In recent years, the trend of infection of Mycobacterium tuberculosis in the world has risen seriously, with about 80 million to 10 million new cases per year, which is the "number one killer" of infectious diseases. The study of prevention, diagnosis and treatment of Mycobacterium tuberculosis has become an urgent task. For a long time, tuberculosis was diagnosed by acid fast staining of sputum or bacterial culture of MTB, combined with clinical symptoms and chest X ray. However, these methods are insensitive due to the growth characteristics of MTB itself, especially for the diagnosis of extrapulmonary tuberculosis. Although ELISA method and dot immunosorbent gold resistance method are used in the screening of tuberculosis, most of the coated antigens are crude antigens, such as polymerized OT,PPD,BCG, hemiglycolipid antigens, which make the results unstable. It is difficult to diagnose extrapulmonary tuberculosis and bacterial culture negative tuberculosis. According to Ivanyi study, the antigens screened from monoclonal antibodies against Mycobacterium tuberculosis were only
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R378

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