4种骨移植材料修复骨缺损动物模型的建立

发布时间：2018-09-01 18:35

【摘要】：目的建立简易骨缺损动物模型,比较珊瑚羟基磷灰石单独及分别与重组人骨形成蛋白2、富血小板纤维、自体骨组合形成3种复合型骨移植材料用于骨缺损修复的效果。方法以6只beagle犬为实验动物,用环形取骨钻在其双侧胫骨干骺端制造4个相同大小的骨缺损,同期在骨缺损区分别充填珊瑚羟基磷灰石(对照组)、珊瑚羟基磷灰石和重组人骨形成蛋白2的复合型骨移植材料(A组)、珊瑚羟基磷灰石和富血小板纤维的复合型骨移植材料(B组)、珊瑚羟基磷灰石和自体骨的复合型骨移植材料(C组)。术后3个月处死所有动物,取材,制作组织切片,HE染色,光学显微镜观察分析。结果 3个月后,6只实验犬均无死亡,饮食、活动正常;组织学观察见各骨缺损内均有大量新生骨组织充填,新旧骨组织有明显界限,新生骨组织的骨小梁不规则。与对照组比较,其他3组的新生骨小梁结构更密集,连续性更好,且这3组之间的区别不明显。结论 3种复合型骨移植材料修复骨缺损均好于单独使用珊瑚羟基磷灰石。
[Abstract]:Objective to establish a simple animal model of bone defect and to compare the effects of three kinds of composite bone graft materials: coral hydroxyapatite alone and with recombinant human bone morphogenetic protein 2, platelet-rich fiber and autologous bone for bone defect repair. Methods six beagle dogs were used as experimental animals. Four bone defects of the same size were made in the bilateral tibial metaphysis with a circular bone drill. At the same time, the composite bone graft materials were filled with coral hydroxyapatite (control group), coral hydroxyapatite and recombinant human bone morphogenetic protein 2 (group A), coral hydroxyapatite and platelet-rich fiber (group A). Bone graft materials (group B), coral hydroxyapatite and composite bone graft materials (group C). All the animals were killed 3 months after operation. Results 3 months later, no death, normal diet and normal activity were found in 6 dogs. Histological observation showed that a large number of new bone tissues were filled in each bone defect, the new and old bone tissues had obvious boundaries, and the trabeculae of new bone tissue were irregular. Compared with the control group, the new trabecular structure of the other three groups was denser and the continuity was better, and the difference between the three groups was not obvious. Conclusion three kinds of composite bone graft materials are better than coral hydroxyapatite alone in repairing bone defect.
【作者单位】：遵义医学院珠海校区研究生院;广东省口腔医院·南方医科大学附属口腔医院种植中心;
【基金】：广东省社会发展领域科技计划项目(2011B080701056)
【分类号】：R318.08;R-332

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