低硒对F344大鼠子代神经行为发育和海马神经前体细胞分化、成熟蛋白表达的影响

发布时间：2018-09-10 05:54

【摘要】：背景与目的硒是人和动物生命活动所必需的微量元素。硒缺乏会引起含硒酶活性降低，氧自由基清除受阻、内分泌紊乱等一系列机体功能障碍，其对神经系统的影响也越来越引起学者们的重视。前期本课题组建立了人工膳食低硒、低碘和联合低硒低碘喂养的SD大鼠动物模型，并历时近三年繁衍至仔四代，研究结果发现，仔三代、四代低硒大鼠已经表现出不同程度体格和神经发育延迟、脑内EGFR/MAPK信号传导通路有一定影响、但Morris水迷宫空间记忆能力没有明显影响等。本实验在前期研究的基础上，再次通过人工低硒饲料喂养F344近交系大鼠复制低硒动物模型，深入细致研究其子代出生后早期不同时间点4d，7d，14d，21d的含硒酶GPx(glutathione peroxidase，谷胱甘肽过氧化物酶)活性变化、早期神经行为发育、子代发育期大鼠开场行为和Morris水迷宫空间记忆能力表现。并通过检测脑神经细胞形态发育、海马神经前体细胞标记蛋白(nestin)、增殖细胞核抗原(PCNA)、胶质细胞分化成熟蛋白——胶质纤维酸性蛋白(glial fibrillary acidic protein，，GFAP)和2’，3’-环核甘酸3’-磷酸二酯酶(2，3-cyclic nucleotide，3-phosphodiesterase，CNPase)的表达情况，从发育，行为，功能到海马局部形态结构和分化、成熟蛋白的表达，进一步深入探讨低硒对早期神经发育的影响，寻找这一影响的形态结构和功能基础。方法 1 复制F344低硒大鼠动物模型参照本课题组已成功建立的人工膳食SD低硒大鼠模型
[Abstract]:Background and objective selenium is an essential trace element for human and animal life. Selenium deficiency can lead to the decrease of the activity of selenase, the elimination of oxygen free radicals, endocrine disorders and a series of functional disorders. The effect of selenium deficiency on nervous system has been paid more and more attention by scholars. In the early stage of the study, the SD rat model of artificial diet with low selenium and iodine and low selenium and iodine was established, which lasted for nearly three years to reproduce to the fourth generation of the offspring. The results showed that the third generation, the third generation. Four generations of low selenium rats have shown different degrees of physical and neurodevelopmental delay, brain EGFR/MAPK signal transduction pathway has a certain effect, but Morris water maze spatial memory ability has no significant effect. On the basis of previous studies, the animal model of selenium deficiency was established by feeding F344 inbred strain rats with artificial low selenium diet. The activity of selenase GPx (glutathione peroxidase, glutathione peroxidase (GPx (glutathione peroxidase,), the early neurobehavioral development, the open field behavior of rats and the spatial memory ability of Morris water maze at different time points of 4 days and 7 days and 14 days after birth were studied. And by detecting the morphological development of brain nerve cells, The expression of (nestin), proliferating cell differentiation maturation protein (PCNA), glial fibrillary acidic protein (glial fibrillary acidic protein,GFAP), and 2Phosphate 3-cyclic nucleotide,3-phosphodiesterase,CNPase (2 ~ 3-cyclic nucleotide,3-phosphodiesterase,CNPase) in hippocampal neural progenitor cells (NPCs) were studied. Behavior, function to hippocampal local morphology and differentiation, mature protein expression, further in-depth study of selenium deficiency on the early neural development, to find the morphological structure and functional basis of this effect. Methods 1 the animal model of F344 low selenium rats was established according to the SD low selenium rat model which was successfully established by our group.
【学位授予单位】：汕头大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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