恒河猴脑选择性超深低温断血流复苏模型的制备

发布时间：2018-09-11 09:15

【摘要】：目的:探讨猴脑选择性超深低温断血流复苏模型的制备方法,规范动物模型。方法:4-10岁健康恒河猴10只,动物随机分为三组:四血管(双侧颈总动脉及椎动脉)阻断冷灌注组,两血管(双侧颈总动脉)阻断冷灌注组,两血管(双侧颈总动脉)阻断等温灌注组。切开双侧颈动脉鞘,结扎右侧颈总动脉,右侧颈内动脉远心端插管连接冷灌注系统,右侧颈内静脉远心端及双侧股静脉近心端插管连接超滤复温装置,建立脑局部体外循环。夹闭双侧颈外静脉、左侧颈内动脉和颈内静脉(四血管阻断冷灌注组还要阻断双侧椎动脉),经右颈内动脉远心端输入4.0±0.5℃冷灌注液(两血管阻断等温灌注组输入38.0℃灌注液),同时,自右侧颈内静脉远心端及右侧股静脉近心端回流静脉血,回流液超滤复温后经左侧股静脉近心端回输至体循环,维持脑温在14.3～16℃之间60min。手术前后行头颅MRI检查,手术中持续行脑电图监护并监测生命体征及血流动力学指标变化,术后每周行神经功能缺失评分,脑组织及主要脏器行光镜观察。结果:在10只实验猴中,两血管阻断冷灌注组4只恒河猴术中、术后血流动力学稳定,灌注60min后安全复苏并长期存活,术后头颅MRI检查未见异常,术后脑电图正常,术后神经功能评分无异常,主要器官组织形态未见异常;两血管阻断等温灌注组2只恒河猴及四血管阻断冷灌注组4只恒河猴于灌注60min后均未能安全复苏,全部死亡,主要器官组织形态可见不同程度异常。结论:猴脑在选择性超深低温状念下,阻断双侧颈总动脉血流60min后可安全复苏,断血流及复苏对血流动力学、脑组织及神经功能无明显影响。
[Abstract]:Objective: to study the method of resuscitation model of selective ultra-deep hypothermia hemodialysis in monkey brain and standardize the animal model. Methods Ten healthy rhesus monkeys aged 4-10 years old were randomly divided into three groups: four vessels (bilateral common carotid artery and vertebral artery) blocking cold perfusion group, two vessels (bilateral common carotid artery) blocking cold perfusion group. Two vessels (bilateral common carotid artery) blocked isothermal perfusion group. Open bilateral carotid sheath, ligate right common carotid artery, intubate distal end of right internal carotid artery to connect cold perfusion system, intubate distal end of right internal jugular vein and proximal heart of bilateral femoral vein to connect with ultrafiltration rewarming device to establish local cerebral cardiopulmonary bypass. Occlusion of bilateral external jugular veins, Left internal carotid artery and internal jugular vein (bilateral vertebral artery were blocked in cold perfusion group by four vessels), 4.0 卤0.5 鈩，

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