新生大鼠神经干细胞定向分化为少突胶质细胞的实验研究
发布时间：2018-09-13 13:14
【摘要】： 第一部分新生大鼠神经干细胞的分离、培养 研究背景及目的神经干细胞(neural stem cells, NSCs)是多分化潜能的细胞,可分化为神经系统的神经元、星形胶质细胞和少突胶质细胞,在胚胎及成年哺乳类动物中广泛存在。NSC的分离培养是其基础和应用研究的前提,本部分目的在于获得新生大鼠NSCs,为深入研究其增殖分化奠定基础。 方法从新生24h内大鼠端脑及中脑部位分离NSC,采用体外无血清悬浮培养,以获得能在体外长期生存的NSC;通过单细胞克隆实验检测其自我更新能力,免疫细胞化学检测NSC标志物nestin表达及分化为神经元和胶质细胞的能力;用细胞计数法研究其生长特点并绘制生长曲线。 结果获得的NSC能在体外长期生存,具有很强的增殖能力、自我更新能力,能分化为神经元和胶质细胞,具有多向分化能力,表达nestin,体外传代至10代细胞数量扩增了40倍。 结论成功地从新生大鼠端脑和中脑中分离得到了能在体外长期
[Abstract]:The first part: isolation and culture of neural stem cells from neonatal rats background and objective Neural stem cells (neural stem cells, NSCs) are multi-differentiated cells. The isolation and culture of neurons, astrocytes and oligodendrocytes, which can differentiate into nervous system, are the premise of the basic and applied research on the separation and culture of .NSC in embryos and adult mammals. The aim of this part is to obtain NSCs, from neonatal rats and lay a foundation for further study of its proliferation and differentiation. Methods NSC, was isolated from rat telencephalon and midbrain within 24 hours and cultured in vitro with serum-free suspension. NSC;, which could survive for a long time in vitro, was tested for its self-renewal ability by single cell clone assay. Immunocytochemistry was used to detect the expression of NSC marker nestin and its ability to differentiate into neurons and glial cells. Results the obtained NSC could survive for a long time in vitro, had strong ability of proliferation, self-renewal, differentiation into neurons and glial cells, and had the ability of multi-directional differentiation. The number of cells subcultured to the 10th passage of the expressed nestin, in vitro was expanded by 40 times. Conclusion\
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329.28
[Abstract]:The first part: isolation and culture of neural stem cells from neonatal rats background and objective Neural stem cells (neural stem cells, NSCs) are multi-differentiated cells. The isolation and culture of neurons, astrocytes and oligodendrocytes, which can differentiate into nervous system, are the premise of the basic and applied research on the separation and culture of .NSC in embryos and adult mammals. The aim of this part is to obtain NSCs, from neonatal rats and lay a foundation for further study of its proliferation and differentiation. Methods NSC, was isolated from rat telencephalon and midbrain within 24 hours and cultured in vitro with serum-free suspension. NSC;, which could survive for a long time in vitro, was tested for its self-renewal ability by single cell clone assay. Immunocytochemistry was used to detect the expression of NSC marker nestin and its ability to differentiate into neurons and glial cells. Results the obtained NSC could survive for a long time in vitro, had strong ability of proliferation, self-renewal, differentiation into neurons and glial cells, and had the ability of multi-directional differentiation. The number of cells subcultured to the 10th passage of the expressed nestin, in vitro was expanded by 40 times. Conclusion\
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329.28
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