益生菌粘附能力评估模型的初步研究
发布时间：2018-09-18 06:52
【摘要】：人体内有许多致病因素会影响肠道正常菌群的生长,从而导致其失调,出现诸如腹痛、腹泻等临床症状。微生态学研究表明益生菌能粘附肠上皮细胞,形成天然生物屏障,显著降低这类疾病的发生率。进入肠道内的益生菌通过粘附定植于肠粘膜表面以保护肠粘膜上皮细胞免受各种病原微生物的损伤,因此益生菌在肠道内的粘附定植是其发挥生理作用的前提和基础。目前,国内外微生态制剂的评估方法有很多,但是缺乏横向比较的资料。因此,本研究试图对比益生菌粘附能力评估模型,以期提供这方面的基础资料。 方法:以培菲康产品菌为模示菌株,采用革兰氏染色、ELISA、放射性同位素标记法和粘附抑制四种模型,分别评估了嗜酸乳杆菌、双歧杆菌、粪肠球菌的粘附能力。通过灵敏度、时间、费用方面的对比,确定最佳的评估模型。采用氯化锂对乳杆菌的S层蛋白进行提取,观察表面蛋白(S层蛋白)对乳杆菌粘附能力评估模型的影响。 结果:益生菌的粘附性能检测结果表明,三种粘附模型对益生菌粘附能力的评估基本一致,革兰氏染色模型相对其他模型更为直观、省时,而且也不需要价格昂贵的分析仪器,可以作为益生菌粘附能力评价标准模型。益生菌的表面(S层)蛋白被抽提以后,其粘附能力被显著影响(P0.01)。因此采用粘附模型评估微生态制剂应尽量避免化学试剂对S层蛋白的影响。 结论:革兰氏染色模型可以用做标准益生菌的粘附能力评估模型,具有灵敏度高,操作简易等特点。益生茵可抑制致病菌对肠上皮样细胞HT-29的粘附和侵袭,减弱致病菌对肠上皮细胞样HT-29的损伤。
[Abstract]:There are many pathogenic factors that affect the growth of normal intestinal flora, leading to its disorders, such as abdominal pain, diarrhea and other clinical symptoms. Microecological studies showed that probiotics could adhere to intestinal epithelial cells and form natural biological barrier, which significantly reduced the incidence of these diseases. Probiotics entering the intestinal tract can protect the intestinal epithelial cells from the damage of various pathogenic microorganisms by adhering to the intestinal mucosal surface, so the adhesion and colonization of probiotics in the intestinal tract is the premise and basis of its physiological function. At present, there are many evaluation methods for microecological agents at home and abroad, but lack of horizontal comparison data. Therefore, this study attempts to compare the evaluation model of probiotic adhesion in order to provide basic data. Methods: the adhesiveness of Lactobacillus acidophilus, Bifidobacterium faecalis and Enterococcus faecalis were evaluated by Gram-stained ELISA, radioisotope labeling and adhesion inhibition. Determine the best evaluation model by comparing sensitivity, time, and cost. The S-layer protein of Lactobacillus was extracted by lithium chloride, and the influence of surface protein (S-layer protein) on the adhesion ability of Lactobacillus was observed. Results: the results of adhesion test of probiotics showed that the three adhesion models were basically consistent in evaluating the adhesion ability of probiotics, and Gram staining model was more intuitive, time-saving, and did not need expensive analytical instruments compared with other models. It can be used as the standard model for evaluating the adhesion ability of probiotics. The adhesion of probiotic surface protein (S layer) was significantly affected (P0.01). Therefore, the effect of chemical reagent on S layer protein should be avoided by using adhesion model to evaluate microecological preparation. Conclusion: Gram-staining model can be used to evaluate the adhesion ability of standard probiotics with high sensitivity and easy operation. Probiotics could inhibit the adhesion and invasion of pathogenic bacteria to intestinal epithelial-like cells (HT-29) and attenuate the damage of pathogenic bacteria to intestinal epithelial-like HT-29.
【学位授予单位】：西南大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R371
本文编号：2247119
[Abstract]:There are many pathogenic factors that affect the growth of normal intestinal flora, leading to its disorders, such as abdominal pain, diarrhea and other clinical symptoms. Microecological studies showed that probiotics could adhere to intestinal epithelial cells and form natural biological barrier, which significantly reduced the incidence of these diseases. Probiotics entering the intestinal tract can protect the intestinal epithelial cells from the damage of various pathogenic microorganisms by adhering to the intestinal mucosal surface, so the adhesion and colonization of probiotics in the intestinal tract is the premise and basis of its physiological function. At present, there are many evaluation methods for microecological agents at home and abroad, but lack of horizontal comparison data. Therefore, this study attempts to compare the evaluation model of probiotic adhesion in order to provide basic data. Methods: the adhesiveness of Lactobacillus acidophilus, Bifidobacterium faecalis and Enterococcus faecalis were evaluated by Gram-stained ELISA, radioisotope labeling and adhesion inhibition. Determine the best evaluation model by comparing sensitivity, time, and cost. The S-layer protein of Lactobacillus was extracted by lithium chloride, and the influence of surface protein (S-layer protein) on the adhesion ability of Lactobacillus was observed. Results: the results of adhesion test of probiotics showed that the three adhesion models were basically consistent in evaluating the adhesion ability of probiotics, and Gram staining model was more intuitive, time-saving, and did not need expensive analytical instruments compared with other models. It can be used as the standard model for evaluating the adhesion ability of probiotics. The adhesion of probiotic surface protein (S layer) was significantly affected (P0.01). Therefore, the effect of chemical reagent on S layer protein should be avoided by using adhesion model to evaluate microecological preparation. Conclusion: Gram-staining model can be used to evaluate the adhesion ability of standard probiotics with high sensitivity and easy operation. Probiotics could inhibit the adhesion and invasion of pathogenic bacteria to intestinal epithelial-like cells (HT-29) and attenuate the damage of pathogenic bacteria to intestinal epithelial-like HT-29.
【学位授予单位】：西南大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R371
【引证文献】
相关博士学位论文 前1条
1 许飞利;我国食品工业常用益生乳酸菌菌种分型与溯源数据库的研究[D];江南大学;2011年
相关硕士学位论文 前1条
1 邹芳;鸡源乳酸菌对肉鸡肠道粘蛋白的粘附性研究[D];东北农业大学;2010年
，本文编号：2247119
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